rDNA序列在多种蔬果类植物染色体上的定位

Localization of rDNA Sequences in a Variety of Vegetable & Fruit Plants on Chromosome

利用改进的rDNA序列标记荧光原位杂交方法,将18S-5S rDNA探针序列直接在5'端进行荧光修饰,并成功将其应用到多种植物染色体上,该方法比常规方法具有省时、经济、快速、信号强等特点,使rDNAFISH杂交更为简单,更易操作.首次利用该技术成功地对30种蔬果类植物中期染色体进行18S-5S rDNA的物理定位,初步了解了rDNA序列在这些蔬果类植物基因组中位点数目和分布特点,其中大蒜、大葱、蚕豆、萝卜(3种)茴香、红菜苔、空心菜、芥兰、莴苣、乌塌菜、雪里蕻、茼蒿、油菜、辣椒、茄子、芹菜、菜豆、花椰菜等20种植物首次确定了18S-5S rDNA在中期染色体上的位置,其余10种植物与前人的结果相吻合.该方法可以作为染色体的一个识别指标,也可为蔬果类植物属内进化和物种分化研究提供重要资料.

rDNA sequences were fluorescence in situ hybridization method,18 S-5 S rDNA probe sequence in the synthesis directly in the 5 ＇end labeled,successfully applied to many plant chromosome,this method than the conventional method Time-saving,economical,fast,signals and other characteristics,so that rDNA-FISH hybridization is more simple,easier to operate. The author first Usingthis way to 30 species of fruits and vegetables successfully among different genera of plants,species chromosome 18 S-5 S rDNA on the physical location,preliminary exploration of the rDNA sequences of those species of vegetables and fruit in the plant genome the number of sites And distribution. 20 kinds of plants garlic,green onions, beans, radish（3） fennel, red Cabbage, spinach, kale, lettuce, Wuta vegetables, Brassica juncea,garland chrysanthemum, rape, pepper, eggplant, Celery, beans, broccoli are the first location.The other10 kinds of plants consistent with the previous results. This study can be used as an identification index of chromosomes, and also provides a clue as vegetables and fruit plants within the evolution and differentiation of species.