北方硕螽不同时期受精囊的组织学结构观察

Structure of the Spermatheca in Different Stages of Deracantha onos Pallas(Orthoptera: Tettigoniidae)

应用光学显微镜和扫描电子显微镜技术对北方硕螽(Deracantha onos Pallas)6龄若虫、未交配和交配成虫3个时期受精囊的组织学结构进行了比较研究。结果显示,6龄若虫受精囊上皮层细胞处于增殖状态,各种细胞的形态不易区分;未交配成虫的受精囊上皮层细胞已初步分化,分布层次不明显,上皮层端部出现颗粒物;已交配成虫的受精囊分化出上皮细胞、腺细胞和导管细胞3种细胞类型,分布层次明显,颗粒物消失,腺细胞分泌腔呈现多态性。此外,已交配受精囊内表面分布有棘和导管细胞的导管开口,其形态均存在区域性差异。研究表明交配活动可能诱导了腺细胞的分泌活性,在受精囊不同区域腺细胞和棘可能行使不同的生理功能。

The structures of the spermatheca in 6th-instar nymphs, unmated and mated females of Deracan- tha onos Pallas （Orthoptera： Tettigoniidae） were investigated by light and scanning electron microscopy. The results showed that epithelial cells of the 6th-instar nymphs were undergoing proliferation, and their mor- phology was not easy to identify. The preliminary differentiation of the epithelial cells could be seen in the unmated adults, but their distribution was not obvious. The granules occurred in the apical cytoplasm of the epithelial layer. In the spermatheea of mated adults, the differentiated cells formed three types of epithelial cells distributed in distinctive layers, and the granules, which appeared in unmated adults, disappeared. The secretory cavities of glandular cells had polymorphism. Furthermore, on the internal surface of the spermath- eta from mated females, the shapes of the bristles and the openings of secretory duct were regionally dis- tributed. It is suggested that mating activity may induce glandular cells to produce large amount of secre- tions. The bristles and glandular cells in varied regions may play different roles and functions in insects.