外周血内IL-37、IL-8、IL-6水平与活动性肺结核患者病情的相关性

Correlation of the levels of interleukin-37,interleukin-8,interleukin-6 in peripheral blood and pathogenic condition of patients with activepulmonary tuberculosis

目的探讨活动性肺结核患者(ATB)在病情进展中外周血IL-37、IL-8、IL-6的表达水平与病情的相关性,为肺结核疾病的防治提供理论依据。方法选取河北省沧州市传染病医院传染科ATB患者78例,非ATB患者54例及健康体检者(HV)56例,采用酶联免疫吸附法(ELISA)检测比较三组患者外周血IL-37、IL-8、IL-6水平。进一步将ATB患者分为空洞组(42例)和非空洞组(36例),胸水组(47例)和无胸水组(31例),对比其炎症因子表达水平。并对132例肺结核患者进行抗结核治疗后的跟踪检测,比较治疗前后相应指标的变化情况。结果外周血IL-6、IL-8、IL-37表达量ATB患者[(36.22±6.84)ng/L,(24.12±5.07)ng/L,(9.65±2.84)ng/L]均显著高于非ATB组[(12.85±4.78)ng/L,(11.55±3.03)ng/L,(7.31±2.11)ng/L]及健康对照组[(2.52±0.31)ng/L,(4.09±0.01)ng/L,(4.20±0.66)ng/L],ATB组与非ATB组比较,差异有统计学意义(P<0.05)。ATB组和非ATB组与健康对照组比较,差异有统计学意义(P<0.05)。132例跟踪患者治疗后外周血IL-6、IL-8、IL-37含量较治疗前均有明显下降(P<0.05)。ATB空洞组IL-37水平与非空洞组比较[(9.79±2.44).(5.27±1.06)ng/L],差异有统计学意义(P<0.05)。胸水组IL-6及IL-8水平与无胸水组比较[(36.07±7.22)vs.(29.87±6.12)ng/L;(27.90±6.35)vs.(20.36±3.13)ng/L]差异有统计学意义(P<0.05)。ATB组IL-6与IL-37表达水平呈正相关(r=0.643,P<0.05),IL-37表达与外周血白细胞总数呈负相关(r=-0.566,P<0.05),与外周血单核细胞呈负相关。结论 IL-37、IL-8、IL-6在ATB患者中具有较高的表达水平,且对病情的判断及评估预后具有一定参考价值,可作为ATB的疗效评估指标。

Objective To investigate the correlation of the levels of IL-37,IL-8,IL-6 and the pathogenic condition of patients with activepulmonary tuberculosis（ATB）,and provide the oretical basic for prevention and cure treatment of tuberculosis. Methods A total of 132 cases of tuberculosis were included in this study,78 of activepulmonary tuberculosis and 54 in resting period;another 56 healthy people were used asnormal control. ELISA method was used to detect the level of IL-37,IL-8,IL-6 in peripheral blood. Patients with ATB were further divided into：cavity group（42 cases）and noncavity group（36 cases）,hydrothorax group（47 cases） and non-hydrothorax group（31 cases）,and the levels of inflammatory factors were compared. Besides,132 patients had been followed up after treatment,and the changes of corresponding indicators were compared before and after treatment. Results The levels of IL-6,IL-8 and IL-37 were（36.22±6.84）ng/L,（24.12±5.07）ng/L and（9.65±2.84）ng/L,respectively,in the patients of activepulmonary tuberculosis,which were significantly higher than those in the patients at resting period[（12.85±4.78）ng/L,（11.55±3.03）ng/L,（7.31±2.11）ng/L]and in normal control[（2.52±0.31）ng/L,（4.09±0.01）ng/L,（4.20±0.66）ng/L]（P〈0.05）.The IL-6,IL-8 and IL-37 levels of the ATB group and non-ATB group were significantly higher than those of the normal control（P〈0.05）. Monitoring on the 132 patients showed that peripheral blood IL-6,IL-8 and IL-37 levels were decreased distinctly after treatment for tuberculosis. The level of IL-37 in cavity group was significantly higher than that of the non-cavity group[（9.79±2.44）vs.（5.27±1.06）ng/L]（P〈0.05）. The levels of IL-6 and IL-8 in hydrothoraxgroup were higher than those in the non-hydrothorax group[（36.07±7.22）vs（.29.87±6.12）ng/L;（27.90±6.35）vs.（20.36±3.13）ng/L],and the difference was statistically significant（P〈0.05）. The level of IL-6 in ATB was positively correlated with the IL-37 level（r=0.643,P〈0.05）.The level of IL-37 in ATB was negatively correlated with the number of white blood cells（r=-0.566,P〈0.05）. Conclusion The patients with ATB had an increase in the levels of peripheral blood IL-37,IL-8 and IL-6,which had certain reference value for the judgment and evaluation of condition,and might be indicators of curative effect in ATB treatment.