姜黄素对百草枯中毒大鼠肺组织胶原面积及TNF-α、IL-6、NE表达的影响

Effects of curcumin on the lung collagen area and the expressions of TNF-α, IL-6 and NE in paraquat-poisoned rats

目的观察姜黄素对百草枯中毒大鼠肺组织胶原面积及TNF-α、IL-6、NE表达的影响，以探讨姜黄素治疗百草枯中毒的可能机制。方法SPF级Wistar大鼠108只，随机（随机数字法）分为空白对照组（B组）、百草枯染毒组（PQ组）、姜黄素治疗组（PC组），PQ组及PC组给予百草枯（50 mg/ kg）（北京华都生物科技）一次性灌胃染毒复制中毒模型，同时空白对照组给予等量生理盐水灌胃；灌胃30 min后，PC组给予姜黄素（sigma公司）200 mg/kg一次性腹腔注射，B组、PQ组注射等体积生理盐水，常规饲养。于3 d、7 d、14 d，行HE染色、Masson染色观察肺组织病理形态学、胶原纤维的分布及变化，酶联免疫吸附法检测血清肿瘤坏死因子（TNF-α）、白介素（IL-6）含量，免疫组织化学法观察肺组织中性粒细胞弹性蛋白酶（NE）的表达及变化。结果与B组比较，PQ组各时间点肺组织均有不同程度的肺泡炎，以7 d时最为严重；肺组织弥漫性纤维化，大量胶原纤维沉积，14 d胶原纤维沉积最严重；NE表达明显增强，14 d最明显。血清TNF-α、IL-6含量亦显著升高（P＜0.05，P＜0.01）；与PQ组比较，PC组各时间点肺泡炎及肺纤维化程度均有明显减轻，NE表达明显减弱，TNF-α、IL-6含量亦显著降低（P＜0.05）。结论抑制炎症因子及NE的表达，降低大鼠的肺泡炎和肺纤维化程度，可能是姜黄素治疗百草枯中毒肺的作用机制之一。

Objective To observe the effects of curcumin on the lung collagen area and the expression of TNF-α, IL-6 and NE in paraquat-poisoning rats at different intervals, and discuss the possible mechanism of curcumin antagonizing paraquat poisoning. Methods A total of 108 SPF Wistar rats were divided into three groups （random number） ： blank group （B group） for control, paraquat poisonin group （PQ group） and cureumin-treatment group （ PC group）. The rats of PQ group and PC group were given paraquat （50 mg/kg） by gavage, and the rats of B group were given equal volume of sterile saline solution at the same time. Thirty minutes later, the rats of PC group were given curcumin （200 mg/kg ） by intraperitoneal injection, and rats of B group and PC group were given equal volume of sterile saline solution instead. At 3 d, 7 d and 14 d after modeling, the distribution and pathological changes of lung tissue and collagen fiber were observed by HE and Masson staining. The concentration of tumor necrosis factor （ TNF-α） and interleukin-6 （IL-6） were detected by enzyme-immunoassay. The lung neutrophil elastase （NE） expression was observed by immuno-histochemical method. Result Compared with B group, PQ group had pulmonary alveolitis in different degrees at different intervals, and the most serious pulmonary alveolitis was observed at 7 d after modeling. Diffused pulmonary fibrosis of the lung tissue and a large area of collagen fiber deposition were observed especially at 14 d after modeling, as well as the expression of NE was observed obviously, especially at 14 d after modeling. The concentration of TNF-α, IL-6 in serum were significantly increased （P 〈 0. 05, P 〈 0. 01 ）. Compared with PQ group, the pulmonary alveolitis and fibrosis obviously in PC group with obvious reduction in the expression of NE and significant descrease in the concentrations of TNF-α and IL-6 （ P 〈 0.05 ）. Conclusion Inhabiting inflammatory factors to alleviate the seriousness of alveolar inflammation and pulmonary fibrosis might be one of the mechanism of treatment with curcumin for paraquat poisoning rats.