MIF、Cyr61在牙龈癌中的表达及其临床意义

Expression of MIF and Cyr61 in Gingival Carcinoma and Potential Clinical Application

【目的】探讨巨噬细胞移动抑制因子（Macrophage Migration Inhibitory Factor,MIF）和高半胱氨酸蛋白61（Cysteine-rich 61,Cyr 61）在牙龈癌组织中的表达及其意义。【方法】选取2012年4月至2016年7月本院收治牙龈癌患者58例的肿瘤组织切片样本,同时随机抽取其中11例患者癌旁正常牙龈组织切片样本。采用免疫组化法检测MIF和Cyr61的表达,并分析其表达与临床病理特征的相关性。【结果】MIF在牙龈癌组织中阳性表达率为70.7%（41/58）,明显高于癌旁正常组织中阳性率36.4%（4/11）;Cyr61在牙龈癌组织中阳性表达率为89.7%（52/58）,明显高于癌旁正常组织中阳性率54.5%（6/11）。MIF与Cyr61的阳性表达与患者的年龄、性别均无相关性（P〉0.05）,而与牙龈癌组织分化程度、患者是否出现淋巴结转移呈显著的正相关; MIF与cyr61两者呈现正相关（r=0.355,P=0.022）。【结论】MIF和Cyr61在牙龈癌组织中高表达,并与分化程度、淋巴转移相关,且两者呈现正相关,其阳性表达可作为牙龈癌在临床上诊断治疗的参考指标。

【Objective】To explore the expression of macrophage migration inhibitory factor （MIF） and cysteine-rich 61 （Cyr 61） in gingival carcinoma and their clinical application.【Methods】A total of 58 patients with gingival carcinoma treated in our hospital from April 2012 to July 2016 were recruited for the study. Tumor tissues from all 58 cases were collected along with 11 cases of randomly picked adjacent normal gingival tissues among those 58 patients. The expression of MIF and Cyr61 was determined by immunohistochemical staining method, and the clinical data and pathological features of patients were summarized.【Results】The positive expression rate of MIF in gingival carcinoma tissues was found to be 70.7% （41/58）, which was significantly higher than that in adjacent normal tissues 36.4%（4/11）. The positive expression rate of Cyr61 in gingival carcinoma tissues was 89.7% （52/58）, which was significantly higher than that in the adjacent normal tissues 54.5% （6/11）. The positive expression of MIF and Cyr61 had no obvious correlation with the age and gender of patients, while they had a significant positive correlation with the differentiation degree of gingival carcinoma and lymph node metastasis. Both MIF and Cyr61 were positive in 37 cases （63.8%）. There was a positive correlation between MIF and Cyr 61 （r=0.355, P〈0.05）.【Conclusion】The expression levels of MIF and Cyr61 are high in gingival carcinoma tissues and they are correlated with tumor differentiation and lymphatic metastasis. MIF and Cyr 61 are positively correlated with each other, which can be used as a reference index for clinical diagnosis and treatment of gingival carcinoma.