福建省登革热监测点白纹伊蚊mtDNA-COI基因遗传多态性研究

Genetic diversity of cytochrome C oxidase subunit Ⅰ genes in Aedes albopictus from dengue surveillance sites in Fujian Province,China

目的探讨福建省登革热监测点白纹伊蚊线粒体细胞色素C氧化酶亚基Ⅰ(mtDNA-COⅠ)基因序列特征和遗传多态性。方法选择福建省登革热监测点2015—2016年送检部分批次白纹伊蚊雌性成蚊,提取基因组DNA,扩增全长mtDNA-COⅠ基因片段并测序,分析基因特征并构建系统发育树。结果 PCR扩增mtDNA-CO Ⅰ基因共12个片段,测序得到的片段长度在1 476bp—1 494bp之间。与白纹伊蚊参比序列相比,同源性为99%,包含9个位点的碱基差异。根据不同单倍体型序列构建种系发育树,发现6个登革热监测点的白纹伊蚊分属3个单倍体型,即H02、H03、H08。结论确定福建省登革热监测点白纹伊蚊mtDNA-COⅠ基因存在9个单核苷酸差异位点,包括H02、H03、H08共3种单倍体型,为福建省虫媒病毒病监测及其防控提供技术支撑。

The mosquito Aedes albopictus is the primary vector for dengue virus transmission in Fujian Province.Despite that active dengue surveillance has been launched in several sites since 2005,the genetic diversity of A.albopictus from these sites remains exclusive.In this study,mosquito pools collected from dengue surveillance sites during 2015-2016 were randomly selected,the full-length mitochondrial cytochrome C oxidase subunit Ⅰ（mtDNA-COⅠ）were amplified and sequenced.Preliminary sequence alignment of 12 amplicons with the reference sequence indicated 99% homology at nucleotide level,due to variations at 9 nucleotide sites.Three haplotypes,namely H02,H03 and H08,were determined based on phylogenetic analysis with72 reference sequences of known haplotypes.These observations facilitate surveillance and control of arboviral diseases in Fujian.