巴马小型猪脾虚痰浊证模型NEI网络相关指标水平变化的实验研究

Experimental Research on Nei Network Related Parameters Changes of Spleen Deficiency and Phlegm Turbidity Bama Mini-Pig

目的:从神经内分泌免疫网络(NEI)角度研究脾虚痰浊证巴马小型猪脑肠肽和神经递质的影响。方法:将巴马小型猪随机分为正常组和模型组,施以每日冲刺跑步训练联合高脂单笼饲养干预,建立小猪脾虚痰浊证模型。应用酶联免疫法(Elisa)测定血清中皮质醇(COR)、血管紧张素Ⅱ(AngⅡ)、醛固酮(ALD)、5-羟色胺(5-HT)、胰高血糖素样肽-1(GLP-1)、乙酰胆碱(Ach)和去甲肾上腺素(NE)水平。结果:与正常组比较,模型组小型猪脑肠肽、神经递质水平发生差异性变化。结论:脾虚痰浊证模型神经内分泌免疫网络中脑肠肽、神经递质失常,提示NEI是客观存在的。某一环节的失衡可能是出现脾虚痰浊证的病理机制之一。

Objective ： To study the perspective of neuroendocrine immune network （NEI） of spleen deficiency and phlegm turbidity Bama Mini - pigs＇brain - gut peptide and neurotransmitter. Methods ： Twelve Bama Mini - pigs were randomly divided into normal group and model group. With daily sprint running training combined high -fat single cage in- tervention, the spleen deficiency and phlegm turbidity model was established. By using enzyme - linked immunoassay （Elisa） ,we determined corticosterone （COR） in serum, angiotensin Ⅱ （ Ang Ⅱ ）, aldosterone （ALD） and serotonin （5 -HT）, glucagon peptide t （GLP- 1 ）, acetylcholine （Ach） and norepinephrine （NE） levels. Results： Compared with normal group, model group Bama Mini -pigs＇brain -gut peptide and neurotransmitter levels had difference changes. Conclusion： Spleen deficiency and phlegm turbidity syndrome model＇s midbrain intestinal peptide and neurotransmitter disorders in neuroendocrine immune network prompt NEI is objectively existed. A link imbalance may be in one of the patho- genesis of spleen deficiency and phlegm turbidity syndrome.