摘要
测定球孢白僵菌Beauveria bassiana分生孢子在重金属镉Cd(Ⅱ)作用下不同时间段超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)及谷胱甘肽-S-转移酶(GST)活性的变化,以探讨球孢白僵菌分生孢子应对重金属胁迫时的生理生化反应。结果表明:Cd(Ⅱ)胁迫初始阶段(2-6 h),球孢白僵菌分生孢子中POD活性出现抑制现象,POD对Cd(Ⅱ)胁迫较敏感。Cd(Ⅱ)胁迫4 h后,GST、SOD、CAT酶活性均显著增加。GST活性在Cd(Ⅱ)胁迫早期酶活变化最为明显,对菌体细胞起到关键的保护作用。后期GST活性较低,因此其保护作用较弱;POD的活性变化则与GST相反,POD在Cd(Ⅱ)胁迫后期对菌体细胞起到关键的保护作用;Cd(Ⅱ)胁迫下,CAT活性稳定且变化不明显但仍高于正常状态下的CAT酶活;SOD始终保持高的酶活性,在这些抗氧化保护酶中,以SOD最为重要。结果表明,抗氧化酶活性的提高与维持是球孢白僵菌耐Cd(Ⅱ)胁迫的重要生理基础。
The effects of heavy metals Cd (Ⅱ) on antioxidant enzyme ( SOD, CAT, POD and GST ) activities of Beauveria bassiana were studied. B. bassiana spores were exposed to Cd (Ⅱ) at different times in order to evaluate the responses of the spores to heavy metal stress. The enzyme activity of POD was decreased at the inception phase of Cd (Ⅱ) stress, and it was more sensitive to Cd (Ⅱ) stress. The enzyme activities of GST, SOD, CAT were significant increased after four hours of Cd (Ⅱ) stress. The change activity of GST was most evident at the early stress of Cd (Ⅱ) , and it may play a key role in the protection of B. bassiana cell. Otherwise, it has no significant effect on cell later on;The change activity of POD was reversed, POD may play a key role in the protection of B. bassiana cell at the late stage of stress;The activity of CAT was stability and did not change significantly, it's still higher than the activity of CAT in the normal state; SOD has always maintained a high enzyme activity. Among these antioxidant protection enzymes, SOD has the greatest importance. This indicted that the improve and maintain in the activities of antioxidant system were one of the physiological basis for the tolerance to Cd (Ⅱ) stress in B. bassiana spores.
出处
《环境昆虫学报》
CSCD
北大核心
2017年第5期992-999,共8页
Journal of Environmental Entomology
基金
国家自然科学基金(31201577)
青岛农业大学高层次人才科研基金(630609)
山东省“泰山学者”建设工程专项经费
关键词
镉胁迫
球孢白僵菌
抗氧化酶系统
土壤修复
Cd (Ⅱ) stress
Beauveria bassiana
antioxidant system
soil remediation