CIK对SGC-7901胃癌移植瘤小鼠模型体内抗肿瘤作用的实验研究

Anti-tumor effects of CIK on SGC-7901 gastric xenograft in mouse model

目的探讨细胞因子诱导杀伤细胞(CIK)对胃癌移植瘤小鼠模型的抗肿瘤作用并探讨其作用机制。方法体内实验选取60只C57BL/6小鼠分为模型对照组、5-Fu组、CIK大剂量、中剂量、小剂量组和正常对照组(n=10),分别治疗周期为4周,比较各组胃癌小鼠治疗期间移植瘤生长情况,测定各组小鼠血清和肿瘤组织MMP-9、VEGF及bFGF水平。结果 1)CIK中剂量组小鼠瘤体积于治疗后1周、2周、3周和4周时均较CIK小剂量组显著减少(P<0.05),CIK大剂量组小鼠瘤体积治疗后1周、2周、3周和4周时均较CIK中剂量组显著减少(P<0.05);2)治疗4周时CIK中剂量组小鼠血清MMP-9、VEGF和bFGF水平均较小剂量组小鼠显著降低(P<0.05),CIK大剂量组小鼠血清MMP-9、VEGF和bFGF水平均较中剂量组小鼠显著降低(P<0.05);3)治疗4周时CIK中剂量组小鼠移植瘤组织MMP-9、VEGF和bFGF mRNA和蛋白水平均较小剂量组小鼠显著降低(P<0.05),CIK大剂量组小鼠移植瘤组织MMP-9、VEGF和bFGF mRNA和蛋白水平均较中剂量组小鼠显著降低(P<0.05)。结论细胞因子诱导杀伤细胞可显著抑制SGC-7901胃癌小鼠模型瘤组织的生长,并且降低血液及癌组织中MMP-9、VEGF和bFGF的表达。

To investigate the anti-tumor effect of cytokine-induced killer cells（CIK） on gastric xenograft in mice and its mechanism, 60 C57 BL/6 mice were selected and divided randomly into model control group, 5-Fu group, CIK high dose group, CIK middle dose, CIK low dose group and normal control group（n=10）. After treatment of 4 weeks, the levels of MMP-9, VEGF and bFGF in serum and gastric xenograft of mice were measured. Data showed that the tumor volume in CIK middle dose group was significantly decreased compared with that in CIK low dose group at 1 week, 2 weeks, 3 weeks and 4 weeks after treatment（P〈0.05）; the tumor volume in CIK high dose group was significantly decreased compared with that in CIK middle dose group at 1 week, 2 weeks, 3 weeks and 4 weeks after treatment（P〈0.05）. The levels of MMP-9, VEGF and bFGF in serum of CIK middle dose group weresignificantly decreased compared with those of CIK low dose group（P〈0.05） 4 weeks after treatment（P〈0.05）; thelevels of MMP-9, VEGF and bFGF in serum of CIK high dose group were significantly decreased compared withthose of CIK middle dose group（P〈0.05） 4 weeks after treatment（P〈0.05）. The mRNA and protein levels ofMMP-9, VEGF and bFGF in the gastric xenograft of mice in CIK middle dose group were significantly decreasedcompared with those in CIK low dose group（P〈0.05） 4 weeks after treatment（P〈0.05）; the mRNA and protein levelsof MMP-9, VEGF and bFGF in the gastric xenograft of mice in CIK high dose group were significantly decreased compared with those in CIK middle dose group（P〈0.05） 4 weeks after treatment（P〈0.05）. Taken together,cytokine-induced killer cells can significantly inhibit the growth of SGC-7901 gastric xenograft in mouse model and can decrease the expression of MMP-9, VEGF and bFGF in blood and cancer tissue.