番石榴根中一个新的三萜类成分

A new triterpenoid from roots of Psidium guajava

为了研究番石榴Psidium guajava根的化学成分,采用柱色谱技术分离纯化,通过波谱数据鉴定了7个乌苏烷型三萜类化合物:2α,3β,6β,23-四羟基乌苏酸-12,20(30)-双烯-28-O-β-D-葡萄糖苷(1)、2α,3β,6β,23-四羟基乌苏酸-12,18-双烯-28-O-β-D-葡萄糖苷(2)、2α,3β,23-三羟基乌苏酸-12,18-双烯-28-O-β-D-葡萄糖苷(3)、nigaichigoside F1(4)、积雪草苷C(5)、2α,3β,6β,19α,23-五羟基乌苏酸-12,18-双烯-28-O-β-D-葡萄糖苷(6)和2α,3β,19α,23-四羟基乌苏酸(7)。化合物1为新化合物,化合物2~6均为首次在此植物中分离得到。对新化合物进行了体外细胞毒活性测定,化合物1浓度为25μmol·L^(-1)对人肝瘤细胞Bel 7402抑制率为52.5%,显示具有较好的体外抑制肿瘤细胞活性。

To investigated the chemical constituents of the roots of Psidium guajava,we isolated seven compounds by silica gel column chromatography.These include oleanane derivatives,2α,3β,6β,23-tetrahydroxylurs-12,20（30）-dien-28-oic acid β-D-glucopyranoside（1）,2α,3β,6β,23-tetrahydroxylurs-12,18-dien-28-oic acid β-D-glucopyranoside（2）,2α,3β,23-trihydroxylurs-12,18-dien-28-oic acid β-D-glucopyranoside（3）,nigaichigoside F1（4）,asiaticoside C（5）,2α,3β,6β,19α,23-pentahydroxylurs-12,18-dien-28-oic acid β-D-glucopyranoside（6）and 2α,3β,19α,23-tetrahydroxylurs-12-en-28-oic acid（7）.Their structures were elucidated on the basis of spectral analysis with reference to the published data.Compound 1 is brand new,compounds 2-6 were first isolated from this plant.The new compound was evaluated for their cytotoxic activity against human hepatoma Bel 7402 in vitro.The results were expressed as the ratio of inhibiting Bel 7402 cells growth by comparing to untreated cells.The new compound（concentration：25 μmol·L^（-1））showed the ratio values of 52.5%.