三七总皂苷对人脐静脉内皮细胞损伤后活性的影响

Panax Notoginseng Total Saponin on Human Umbilical Vein Endothelial Cell Injury After the Activity

目的:检测三七总皂苷对人脐静脉内皮细胞损伤后的影响,探索三七总皂苷对内皮细胞的保护作用。方法:把体外培养的人脐静脉内皮细胞株传代培养,共分为6组,即正常组,模型组,三七总皂苷4个浓度组,用10μg·L^(-1)的肿瘤坏死因子-α(tumor necrosis factor,TNF-α)造成细胞损伤模型,三七总皂苷组分别加入不同浓度的三七总皂苷2.5 mg·L^(-1)、5 mg·L^(-1)、10 mg·L^(-1)、20 mg·L^(-1),用CCK-8方法检测各组细胞活性。结果:CCK-8检测结果显示,人脐静脉内皮细胞在TNF-α诱导损伤后,用4个浓度的三七总皂苷干预,随着时间的延长,细胞存活率逐渐降低,组间差异具有统计学意义,其中6 h的细胞存活率最高,24 h的细胞活性最差,差异无统计学意义;和模型组相比,三七总皂苷4个浓度组细胞活性明显较高(P<0.01或P<0.05),其中5 mg·L^(-1)三七总皂苷组细胞活性最高,与模型组相比,差异具有统计学意义(P<0.01)。结论:三七总皂苷能提高人脐静脉内皮细胞损伤后的活性。

Objective： Detection of notoginseng total saponin on human umbilical vein endothelial cells after injury and the effect of exploration notoginseng total saponin on protection of endothelial cells. Methods： The in vitro cultured human umbilical vein endothelial cell line subculture,which is divided into six groups,namely the normal group,model group,notoginseng total saponin concentrations of four groups,with 10 mg·L-1of tumor necrosis factor alpha（ TNF-α） cells damage model,notoginseng total saponin group respectively in different concentrations of total saponins of panax notoginseng 2. 5 mg·L-1,5 mg·L-1,10 mg·L-1,20mg·L-1,CCK-8 method was used to inspect each cell activity. Results： CCK-8 test results showed that human umbilical vein endothelial cell injury induced by TNF-α,with notoginseng total saponin of four concentration,with the extension of time,the survival rate of cells decreased gradually,with a significant difference between the groups,of which 6 h cell survival rate was the highest,24 h cell activity difference was the worst,with no statistically significant; compared with the model group,group activity of Notoginseng Total Saponin 4 concentration was significantly higher（ P 〈0. 01 or P 〈0. 05）,the total saponins of 5 mg·L-1concentration group were the highest activity,compared with the model group,the differences were statistically significant（ P 〈0. 01）.Conclusion： Panax notoginseng total saponin can improve the activity of human umbilical vein endothelial cell after injury.