2型糖尿病患者外周血单核细胞亚群脂蛋白相关磷脂酶A2基因表达研究被引量：7

Expression of LP-PLA2 mRNA in peripheral blood monocytes subsets of patients with type 2 diabetes mellitus

导出

摘要目的通过检测2型糖尿病患者外周血单核细胞亚群脂蛋白磷脂酶A2（LP-PLA2）mRNA表达，探讨单核细胞亚群在2型糖尿病发病中的作用。方法收集32例2型糖尿病患者和22名健康对照者外周血，流式细胞仪检测单核细胞亚群比例，PCR检测单核细胞亚群LP-PLA2 mRNA表达。健康人单核细胞亚群在体外诱导为巨噬细胞，经2型糖尿病患者血清刺激24h后检测LP-PLA2 mRNA表达。结果与对照组相比，2型糖尿病患者经典型单核细胞比例降低，非经典型、中间型单核细胞比例及其LP-PLA2 mRNA水平显著升高（P〈0.05）；2型糖尿病患者稳态模型评估的胰岛素抵抗指数与非经典型（P〈0.05）和中间型单核细胞（P〈0.01）LP-PLA2 mRNA水平呈正相关；伴颈动脉粥样硬化2型糖尿病患者与单纯糖尿病患者相比，中间型和非经典型单核细胞LP-PLA2 mRNA水平明显升高（P〈0.05）。巨噬细胞经2型糖尿病患者血清刺激后，非经典型单核细胞LP-PLA2 mRNA水平高于对照组（P〈0.05）。结论2型糖尿病患者外周血单核细胞向中间型和非经典型极化，且高表达LP-PLA2 mRNA，可能参与2型糖尿病和动脉粥样硬化的发生。 Objective To investigate the role of monocyte subsets in the pathogenesis of type 2 diabetes mellitus（T2DM）, the expression was detected on lipoprotein-associated phospholipase A2（LP-PLA2）mRNA in peripheral blood monocytes subsets of type 2 diabetic patients. Methods After the peripheral blood of 32 type 2 diabetic patients and 22 healthy controls were collected, the percentages of the monocytes subsets were detected by flow cytometry. The expression of LP-PLA2 mRNA in monocytes subsets were detected by PCR. After monocyte subsets of healthy subjects were induced into macrophages and stimulated by the serum of type 2 diabetic patients for 24h, LP-PLA2 mRNA expression was detected.Results Compared with the control group, the percentage of classical monocytes in T2DM group was decreased, while the LP-PLA2 mRNA expression and percentage of nonclassical, intermediate monocytes were significantly increased（all P〈0.05）. In addition, the homeostasis model assessment for insulin resistance index was positively correlated with LP-PLA2 mRNA expression in non-classical（P〈0.05）and intermediate monocytes（P〈0.01）of type 2 diabetic patients. Compared with type 2 diabetic patients without carotid atherosclerosis, the levels of LP-PLA2 mRNA in intermediate and nonclassical monocytes were significantly higher than those in type 2 diabetic patients with carotid atherosclerosis（P〈0.05）. The level of LP-PLA2 mRNA in non-classical monocytes-derived macrophages stimulated with the serum of type 2 diabetic patients was significantly higher than that in the control group（P〈0.05）.Conclusion Monocytes subsets from type 2 diabetic patients are abnormally skewed toward to intermediate and non-classical monocytes with high expression of LP-PLA2 mRNA, which may play an important role in the pathogenesis of type 2 diabetes mellitus and atherosclerosis.

作者孙明忠周中卫季禹乔朱晖江冬梅陈红梅

机构地区东南大学医学院附属盐城医院检验科东南大学医学院附属盐城医院内分泌科

出处《中华内分泌代谢杂志》 CAS CSCD 北大核心 2017年第10期822-827,共6页 Chinese Journal of Endocrinology and Metabolism

关键词糖尿病 2型单核细胞亚群脂蛋白相关磷脂酶A2 动脉粥样硬化 Diabetes mellitus, type 2 Monocyte subsets Lipoprotein phospholipase A2 Atherosclerosis

分类号 R446.1 [医药卫生—诊断学] R587.1 [医药卫生—内分泌]

引文网络
相关文献

参考文献2

1李珊珊,殷悦,贾玫.脂蛋白相关磷脂酶A2酶活性水平及其基因多态性与冠心病的相关性研究[J].中华检验医学杂志,2012,35(10):899-904. 被引量：12
2林秀红,徐明彤,麦梨芳,唐菊英,王晓艺,李焱,严励.新诊断2型糖尿病患者血浆脂蛋白相关性磷脂酶A2及分泌型磷脂酶A2水平与动脉粥样硬化的相关研究[J].中华内分泌代谢杂志,2016,32(6):470-474. 被引量：22

二级参考文献40

1Six DA, Dennis EA. The expanding superfamily of phospholipase A ( 2 ) enzymes: classification and characterization. Biochim Biophys Acta,2000,1488 : 1-19.
2Tsimikas S, Tsironis LD, Tselepis AD. New insights into the role of lipoprotein ( a ) -associated lipoprotein-associated phospholipase A2 in atherosclerosis and cardiovascular disease. Arterioscler Thromb Vasc Biol,2007,27:2094-2099.
3Theilmeier G, De Geest B, Van Veldhoven PP, et al. HDL-associated PAF-AH reduces endothelial adhesiveness in apoE-/- mice. FASEB J,2000,14:2032-2039.
4Karabina SA, Elisaf M, Bairaktari E, et al. Increased activity of platelet-activating factor acetylhydrolase in low-density lipoprotein subfractions induces enhanced lysophosphatidylcholine production during oxidation in patients with heterozygous familial hypercholesterolaemia. Eur J Clin Invest, 1997,27:595-602.
5Murakami M, Kudo I. Phospholipase A2. J Biochem,2002,131: 285 -292.
6Marathe GK, Zimmerman GA, Mcintyre TM. Platelet-activating factor acetylhydrolase, and not paraoxonase-1, is the oxidized phospholipid hydrolase of high density lipoprotein particles. J Biol Chem,2003,278:3937-3947.
7Koenig W, Twardella D, Brenner H, et al. Lipoprotein-associated phospholipase A2 predicts future cardiovascular events in patients with coronary heart disease independently of traditional risk factors, markers of inflammation, renal function, and hemodynamic stress. Arterioscler Thromb Vasc Biol, 2006,26 : 1586-1593.
8Sabatine MS, Morrow DA, O'Donoghue M, et al. Prognostic utility of lipoprotein-associated phospholipase A2 tor cardiovascular outcomes in patients with stable coronary artery disease. Arterioscler Thromb Vasc Biol,2007,27:2463-2469.
9Ross R. Atherosclerosis-an inflammatory disease. N Engl J Med, 1999,340 : 115-126.
10Guerra R, Zhao B, Mooser V, et ah Determinants of plasma platelet-activating factor acetylhydrolase : heritability and relationship to plasma lipoproteins. J Lipid Res, 1997,38 : 2281 - 2288.