IL-35对急性髓系白血病细胞增殖和凋亡的影响

Effect of IL-35 on Proliferation and Apoptosis of AML Cells

目的探讨IL-35对急性髓系白血病(Acute Myeloid Leukemia,AML)细胞增殖和凋亡的影响。方法选取3例成人初诊AML患者的骨髓标本,使用流式细胞术(Flow Cytometry,FCM)分选原始细胞,IL-35处理AML原始细胞以及NB4细胞,FCM检测细胞凋亡,CCK8检测细胞增殖。结果 (1)IL-35显著上调AML细胞株中IL-35R的表达,同时还明显促进AML细胞株的增殖,进一步研究发现,IL-35预刺激的AML细胞株能显著抵抗阿糖胞苷(Ara-C)诱导的凋亡;(2)IL-35显著上调AML细胞中IL-35R的表达,同时显著促进AML细胞的增殖,而凋亡实验也发现IL-35预刺激的AML细胞能显著抵抗Ara-C诱导的凋亡。结论抑制性细胞因子IL-35可通过直接促进AML细胞增殖和抑制AML细胞的凋亡来参与AML的发生发展。

Objective To investigate the effect of IL-35 on proliferation and apoptosis of AML cells. Methods Bone marrow specimens of 3 adult ND AML patients were enrolled into this study. Primary cells were sorted by FCM,AML original cell and NB4 cells were treated by IL-35. Apoptosis was detected by FCM,and cell proliferation was detected by CCK8. Results（ 1） IL-35 significantly up-regulated the expression of IL-35 R in AML cell lines,and it also promoted the proliferation of AML cell lines. Further studies showed that AML cell lines pre-stimulated by IL-35 can significantly resist apoptosis induced by Ara-C.（ 2） IL-35 significantly up-regulated the expression of IL-35 R in AML cells,and significantly promoted the proliferation of AML cells. Apoptosis experiments also showed that AML cells pre-stimulated by IL-35 could significantly resist apoptosis induced by Ara-C. Conclusion The inhibitory cytokine IL-35 can participate in the development of AML by directly promoting the proliferation of AML cells and inhibiting the apoptosis of AML cells.