圈养白虎H5N1亚型禽流感病毒的分离鉴定及其HA和NA基因的生物信息分析

Identification of H5N1 subtype avian influenza virus from captive Panthera tigris and analysis of HA and NA gene

采集某动物园病死白虎(Panthera tigers)的组织样本,置于灭菌的PBS中充分研磨,离心后取上清液并加入双抗后,接种于9~10日龄鸡胚,分离到1株具有血凝性的病毒。HA和HI试验表明该病毒为H5亚型禽流感病毒;HA和NA基因序列分析表明该病毒为H5N1亚型禽流感病毒,其HA基因属于Clade 2.3.2.1分支。结果表明:该虎感染了H5N1亚型禽流感病毒,本试验对我国圈养野生虎流感疾病的防控具有重要作用。

Influenza A viruses belongs to the genus strains of sweat virus, which is threatening the health of humans and animals, resulting in plenty of economic loss and increasing the burden on society. In this study,the tissue samples of died Panther tigris were collected and grinded in PBS. After centrifugation,the supernatant was added with double antibody and inoculated in 9-10 days of age chicken embryo,the virus were isolated. The experiments of HA and HI showed that the virus was H5 subtype avian influenza virus;the gene sequence analysis of HA and NA showed that the virus was HSN1 subtype avian influenza virus, the HA gene belongs to the branch of Clade 2.3.2.1. The result indicated thatthe Panther tigers wasinfected by HSN1 subtype highly pathogenic influenza. This study is significant for controlling the influenza disease of captive panthera tigers.