神经元上集落刺激因子受体表达的发生学变化

The ontogenic changes of colony stimulating factor-1 receptor(CSF-1R) expression on neurons

目的 观察小鼠脑神经元集落刺激因子 1受体 (CSF 1R)表达的发生学变化。方法 用免疫组化和免疫蛋白电泳的方法检测不同胎龄至生后 4周小鼠脑内神经元上CSF 1R的表达状况。结果 胎龄 14d小鼠脑神经元上即可观察到CSF 1R的表达 ,并随胎龄增长而逐渐增强。出生时至峰值 ,生后逐渐降低 ,生后 4周达到成年鼠水平。结论 CSF 1R表达水平的消长与脑神经元发生、发展过程具平行关系 。

Objective To observe the ontogenic changes of exprtession of colony stimulating factor 1 receptor(CSF 1R) on neurons.Methods The expression of CSF 1R on neurons was shown by the use of immunochemical staining and immunoblotting(western blot).Results The expression of CSF 1R could be detected on cranial neurons of mice from fetal age 14 days in our experiment.The expression decreased gradually after birth, and keep a plateau after postnatal 4 weeks.Conclusion There is a close relationship between the level of CSF 1R expression and the ontogeny and development of neurons in CNS(central neurons system)in mice.It was suggested that expression of CSF 1R might play an important role in the development course of neural system in mice