铜离子配位甲烷氧化菌素功能化纳米金模拟过氧化物酶的研究

Study on Functionalized Gold Nanoparticles of Methanobactin by Copper Ion Coordination Uesd as Simulated Peroxidase

合成了纳米金-甲烷氧化菌素(Mb)-铜配合物,该配合物可以作为模拟过氧化物酶用于催化过氧化氢氧化对苯二酚的反应.通过紫外光谱、荧光光谱、红外光谱对纳米金-甲烷氧化菌素(Mb)-铜进行了表征.利用紫外-可见分光光度法研究了配合物催化过氧化氢氧化对苯二酚的动力学.考察了体系p H、体系温度及过氧化氢/催化剂摩尔比对催化反应速率的影响.结果表明纳米金-甲烷氧化菌素(Mb)-铜配合物符合生物催化剂条件影响的一般规律,但比生物酶具有更高的热稳定性.

In this paper, AuNPs-methanobactin（Mb）-copper complexes have been synthesized, which can be used as a simulated peroxidase in the catalytic oxidation of hydroquinone by H202. AuNPs-methanobactin（Mb）-copper was characterized by UV spectroscopy, fluorescence spectroscopy and infrared spectroscopy. The kinetics of hydro- quinone oxidation catalyzed by complexes were studied by UV-Vis spectrophotometry. The effects of system pH, system temperature and hydroquinone/complex molar ratio on the catalytic reaction rate were investigated. The re- sults showed that the AuNPs-methanobaetin（Mb）-copper complex conformed to the general rule of the influence of the biocatalyst condition, but having higher thermal stability than the biological enzyme.