聚乳酸非织造材料可生物降解环境中菌群结构分析及调控

Analysis and Regulation of Bacterial Flora in Biodegradable Environment of PLA Nonwoven Materials

为建立非织造材料可生物降解性能的快速检测方法,选择土壤这一自然降解环境介质进行生物降解试验。通过对富集土壤中的混合菌群液配制成生物快速降解环境,运用平板计数和PCR-DGGE技术对该环境中降解前后的细菌菌群结构变化进行分析。结果表明,所富集的土壤细菌混合菌群不仅在传代转接过程,还是在整个生物降解试验周期中,优势菌的数量和种类始终保持相对稳定,确保生物降解的持续性。同时根据对非织造材料生物降解过程中的无机碳含量检测分析,验证了在整个降解周期中细菌菌群发生生物降解的有效性;并通过调节不同浓度的降解试验菌液,可以调控生物降解的速度,从而实现不同的生物降解效果。

Rapid detection method for biodegradability biodegradability of nonwoven materials was developed. Soli was chosen as natural degradation medium for biodegradation experiment. Rapid biodegradation condition was prepared through enrichment of mixed bacteria in soli. In the condition, the change of bacterial community structure before and after biodegradation was analyzed by plate count and PCR-DGGE （ polymerase chain reaction denaturing gradient gel electrophoresis） method. The result showed that the quantity and species of dominant bacteria in mixed flora from soli has remained relatively stable during the successive passage and the whole biodegradation cycle. The concentration of inorganic carbon was detected and analyzed during the biodegradation process of nonwoven materials. It proved an effectively biodegradable of bacterial community during the cycle. It has been achieved different effects to regulate the biodegradation rate by adjusting the different concentration of bacterial community for biodegradation experiment.