放置时间、温度对新生儿末梢血细胞计数的影响分析

Effect of time and temperature on count of peripheral blood cells in newborns

目的 :研究放置时间、温度对新生儿末梢血细胞计数的影响。方法 :收集2016年1—6月间河北北方学院附属第一医院新生儿科收治入院新生儿的末梢血标本100例,选用Sysmex XS-500i全自动血细胞分析仪即时检测后分装置于4℃及室温保存,并于24、48 h后重复测定,比较白细胞(WBC)、红细胞(RBC)、血小板(PLT)、血红蛋白(HGB)、未成熟粒细胞百分比(IG%)及未成熟粒细胞绝对值(IG#)各组数据差异。结果:室温条件下放置24、48 h的新生儿末梢血标本WBC、RBC、PLT、HGB、IG%及IG#与即时检测结果均存在统计学差异(P<0.05),4℃条件下放置24 h的标本与即时检测结果不存在统计学差异(P>0.05),放置48 h的标本与即时检测结果则存在统计学差异(P<0.05)。结论:新生儿末梢血可置于4℃条件下保存,在24 h内检测不影响WBC、RBC、PLT、HGB、IG%及IG#计数准确性。

Objective To study the effect of time and temperature on the count of peripheral blood cells in newborns.Methods The peripheral blood of 100 cases of newborns who were admitted in the First Affiliated Hospital of Hebei North University from January 2016 to June 2016 were collected, and measured with Sysmex XS-500 i automatic blood cell analyzer immediately, then were split into two parts and stored at 4 ℃ and room temperature respectively. The measurement was repeated in 24 and 48 h, and the groups were compared on WBC, RBC, PLT, HGB, IG% and IG#. Results There were statistically significant differences between the instantly measuring result and those in 24 and 48 h under room temperature(P<0.05). In case of 4 ℃, the instantly measuring result had no obvious difference with that in 24 h(P>0.05), while statistical difference with that in 48 h(P<0.05). Conclusion Newborns' peripheral blood can be stored at 4 °C, and the counting results will not be affected for WBC, RBC, PLT, HGB, IG% and IG# within 24 h.