T-SPOT.TB快速诊断脊柱结核的可行性研究

Feasibility Study of T-SPOT.TB in the Rapid Diagnosis of Spinal Tuberculosis

目的:探究T-SPOT.TB试剂盒检测在快速诊断脊柱结核患者中的可行性。方法:选取某院2014年4月~2016年9月入院治疗的脊柱结核患者50例作为A组,同期入院治疗的非脊柱结核患者50例作为B组,同期入院体检健康者50例作为C组。3组患者分别进行结核感染T细胞检测试验、PPD试验和抗结核抗体试验,比较3种检测方法的阳性率、特异度以及敏感度。结果:A组T-SPOT.TB检测方法的阳性率、特异度、敏感度均显著高于PPD和抗结核抗体检测(P<0.05);T-SPOT.TB检测方法的B组和C组阳性率均低于A组(P<0.05);B组3种检测方法的阳性率、特异度、敏感度差异没有统计学意义(P>0.05)。结论:T-SPOT.TB试剂盒诊断脊柱结核患者具有较高特异度和敏感度,在脊柱早期结核分支杆菌感染诊断中具有可行性。

Objective:To investigate the feasibility of T-SPOT.TB kit detection in the rapid diagnosis of spinal tuberculosis.Methods:50 patients with spinal tuberculosis treated in a hospital from April 2014 to September 2016 were selected as group A,while another 50 patients without spinal tuberculosis were selected as group B,and 50 healthy people were selected as group C at the same time.All subjects in the three groups received T cell test of tuberculosis,PPD test and anti-tuberculosis antibody test,and the positive rate,specificity and sensitivity of three groups were compared.Results:The positive rate,specificity and sensitivity of TSPOT.TB detection in group A were significantly higher than those of PPD and anti-tuberculosis antibody test(P<0.05).The positive rates of T-SPOT.TB detection in group B and group C were lower than that of group A(P<0.05).There was no significant difference in the positive rate,specificity and sensitivity among the three detection methods in group B(P>0.05).Conclusion:The T-SPOT.TB kit has high specificity and sensitivity in the diagnosis of spinal tuberculosis,and it is feasible to diagnose mycobacterium tuberculosis infection in the early stage of spinal column.