白藜芦醇对糖尿病大鼠视网膜病变的影响

The effects of Resveratrol on diabetic rats with retinopathy

目的观察白藜芦醇(Resveratrol,RES)对糖尿病大鼠视网膜病变的影响并探讨相关机制。方法清洁级健康雄性SD大鼠45只随机分为对照组、模型组和RES组各15只。应用链脲佐菌素腹腔注射制作糖尿病大鼠模型。造模后1周,RES组大鼠给予白藜芦醇40mg/kg(10mL/kg)腹腔注射治疗,1次/d,连续治疗6周。治疗结束后,测量各组大鼠血糖、体质量,采用酶联免疫吸附测定法检测大鼠视网膜中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)和白细胞介素1β(interleukin-1β,IL-1β)含量,检测大鼠血清中血管内皮生长因子(vascular endothelial growth factor,VEGF)、缺氧诱导因子1α(hypoxia inducible factor-1α,HIF-1α)、丙二醛(methane dicarboxylic aldehyde,MDA)和超氧化物歧化酶(superoxide dismutase,SOD)含量。结果模型组和RES组血糖高于对照组,体质量低于对照组(P<0.05);模型组与RES组血糖、体质量差异均无统计学意义(P>0.05)。模型组和RES组大鼠TNF-α、IL-1β、VEGF、HIF-1α和MDA表达明显高于对照组,RES组大鼠TNF-α、IL-1β、VEGF、HIF-1α和MDA表达低于模型组,差异均有统计学意义(P<0.05)。模型组和RES组大鼠SOD表达明显低于对照组,RES组大鼠SOD表达高于模型组,差异均有统计学意义(P<0.05)。结论 RES可抑制炎症反应和HIF-1α/VEGF通路,减轻氧化应激损伤,从而改善糖尿病视网膜的血管病变。

Objective To observe the effects of Resveratrol(RES)on diabetic rats with retinopathy,and to explore its mechanism.Methods Forty-five cleaning degree healthy male SD rats were randomly divided into 3 groups,control group,model group and RES group(n=15/group).The diabetic rats model were established by streptozotocin intraperitoneal injection.One week after modeling,rats in RES group was administrated intraperitoneally with RES(40 mg/kg,10 mL/kg)once daily for consecutive 6 weeks.After treatment,the blood glucose and body weight of different groups were measured.The expression of tumor necrosis factor alpha(TNF-α)and interleukin-1β(IL-1β)in retina of different groups were detected by enzyme linked immunosorbent assay(ELISA).The expression of vascular endothelial growth factor(VEGF),hypoxia inducible factor-1α(HIF-1α),methane dicarboxylic aldehyde(MDA)and superoxide dismutase(SOD)in serum of different groups were detected by ELISA.Results The blood glucose of model group and RES group was higher than control group,body weight of model group and RES group was lower than control group(P<0.05),there were no significantly difference between model group and RES group(P>0.05).The expressions of TNF-α,IL-1β,VEGF,HIF-1αand MDA in model group and RES group were higher than that of control group.The e expressions of TNF-α,IL-1β,VEGF,HIF-1αand MDA in RES group were lower than that of model group(P<0.05).The expression of SOD in model group and RES group was lower than that of control group.The expression of SOD in RES group was higher than that of model group(P<0.05).Conclusion RES could inhibit inflammatory reaction,suppresses HIF-1α/VEGF pathway and reduces oxidative stress injury,then ameliorates vascular lesions in diabetic retina.