miR-221通过靶向PDCD4影响肾细胞癌发生发展的机制研究

The mechanism of miR-221 affecting the development of renal cell carcinoma by targeting PDCD4

目的研究miR-221在肾细胞癌中靶向PDCD4影响肾细胞癌发生发展的机制。方法取生长状态良好的A498细胞,用lipo2000进行转染,实验组转染miR-221模拟物(mimics),对照组转染空白的miRNA。利用基因芯片对收集的肾细胞癌组织及癌旁组织进行检测;通过MTT和侵袭实验检测过表达miR-221对肾细胞癌细胞增殖能力和侵袭的影响;通过q-PCR检测下游靶基因PDCD4的表达情况。结果与癌旁组织相比,miR-221在肾细胞癌组织中表达明显升高;与对照组比较,实验组过表达miR-221后能够促进肾细胞癌细胞的增殖能力和侵袭能力,使PDCD4表达降低。结论 miR-221可以通过靶向PDCD4影响肾细胞癌细胞A498的增殖和侵袭能力,从而影响肾细胞癌的发生发展。

Objective To investigate the role of miR-221 in the development of renal cell carcinoma to provide evidence for the clinical treatment of renal cell carcinoma. Methods This experiment using gene chip to detect the difference of the level of gene expression in renal cell carcinoma and adjacent tissues. The effect of miR-221 on the proliferation and invasion of renal cell carcinoma cells was detected by MTT assay and invasion assay. The expression of PDCD4 was detected by q-PCR; the expression of qPCR downstream target gene PDCD4 detection. Results Compared with the adjacent tissues,the expression of miR-221 in renal cell carcinoma tissues was significantly increased,overexpression of miR-221 could promote the proliferation and invasion of renal cell carcinoma. The role of miR-221 in renal cell carcinoma was related to PDCD4. Conclusion miR-221 can affect the proliferation and invasion of A498 cells by targeting PDCD4,which may affect the development of renal cell carcinoma.