辣椒遵辣一号与其野生祖先种Chiltepin BZR转录因子家族的特征

Characteristics of BZR transcription factor family of sweet pepper Zunla-1 and its' wild ancestor chiltepin

使用Local BLAST、系统进化分析、蛋白质保守结构域分析等生物信息学工具,笔者筛选出了辣椒重要栽培品种遵辣一号及其野生祖先种Chiltepin(C.annuum var.glabriusculum)基因组中的BZR转录因子成员,并对其系统进化和可能的生物学功能进行分析。结果显示:在系统进化分析中找出了与拟南芥BZR1/BZR2最同源的辣椒基因;辣椒遵辣一号与其野生祖先种Chiltepin基因组中BZR家族都比拟南芥多出2个BZR基因,这2个基因在系统进化树中单独形成1个分支,除BZR转录因子特征性的N末端外,这2个基因还有额外的糖苷水解酶家族14(Glycoside hydrolase family 14,PF01373)结构域;辣椒栽培品种遵辣一号与其野生祖先种Chiltepin的BZR基因序列和染色体位置都较保守,但也有区别,其中,遵辣一号Capana01g004209与Chiltepin Capang08g001073序列虽一致性为98%,但却从Chiltepin的八号染色体变成了遵辣一号中的一号染色体。

Brassinolide（BR） plays an important role in plant growth and development.The BZR transcription factors are key regulators of brassinosteroid signaling.Using a variety of bioinformatics tools,we identified members of the BZR transcription factor in the pepper Zunla-1,an popular commercial cultivar,and Chiltepin（C.annuum var.glabriusculum） genome,the wild ancestor of Zunla-1.Phylogenetic analysis revealed the most closely related peppet BZR genes to Arabidopsis thaliana BZR1/BZR2;The BZR family of the Zunla-1 and its wild ancestor Chiltepin genome had two unique BZR genes.These two genes form a unique branch in the phylogenetic tree,and there is no corresponding gene in the Arabidopsis genome.In addition to the characteristic N-terminus of the BZR transcription factor,the two genes have additional glycoside hydrolase family 14（PF01373） domain;The sequence and chromosomal locations of these BZR genes of Zunla-1,and its wild ancestor,are very conservative.The identity of the Zunla-1 Capana01g004209 and Chiltepin Capang08g001073 sequences was 98%,but Capana01g004209 locates on chromosome one of Zunla-1,while Capang08g001073 locates on the chromosome eight of Chiltepin.