梯度平板法定量分析啤酒污染细菌的四氢异-α-酸抗性

Quantitative test of tetrahydro-iso-α-acid resistance of beer spoilage bacteria using hoptetrahydro-iso-α-acid-gradient medium

快速定量分析啤酒污染细菌的酒花抗性和腐败啤酒能力,对啤酒的工业生产具有重要的应用价值。该研究首先优化并建立了基于酒花苦味物质四氢异-α-酸梯度平板的定量分析方法,并通过该方法定量分析23株啤酒污染细菌的四氢异-α-酸最小抑制浓度、比较分析液体培养基中的生长情况和菌株的腐败啤酒能力。研究结果表明,酒花苦味物质四氢异-α-酸的浓度梯度平板法,能够简单快速并定量分析啤酒污染细菌的四氢异-α-酸抗性,同时能够评估菌株的腐败啤酒能力。

Rapid and quantitative detection of hop resistance and beer spoilage capability in beer spoilage bacte- ria can effectively guide the beer manufacture of breweries. In this study, a new culture-dependent rapid test method based on hop tetrahydro-iso-ct-acid-gradient medium plate was constructed. 23 beer spoilage bacteria strains were used to test the accuracy of method. The minimum inhibitory concentrations of tetrahydro-iso-α-acid, a compound of hop bitter acid, were rapidly detected by the method. The growth capabilities in MRS broth contained tetrahydro-iso-α- acid and 12~P beer broth were compared. These results indicate that the method based on hop tetrahydro-iso-α-acid- gradient plate quite rapid and simple to quantitatively assay hop resistance of beer spoilage bacteria, meantime, evalu- ate the beer spoilage capability.