微生物扩增子高通量测序技术在水产品加工与贮藏中的应用

High-throughput microbial rRNA amplicon sequencing in the study of processing and storage of aquatic food products

简介了微生物扩增子高通量测序技术,简述该技术在水产品加工与贮藏研究中的应用现状。在发酵水产品、水产品低温加工和保鲜防腐等方向,众多研究以高通量方法测定细菌和古菌的16S rRNA基因片段,准确获得水产品中微生物的种类和数量信息。微生物扩增子高通量测序技术优于传统的可培养法,也优于许多非培养分析技术。同时还指出了该技术的存在问题及对策,并探讨了该技术的发展方向和应用前景。

Food microbiology has been revolutionized by the advances in molecular genetic technology of microbi- al ecology. Novel microbial detection methods have been applied in the study of processing and storage of aquatic food products. The microbial diversity in the sample to be studied could be detected by high-throughput sequencing （HTS） approaches as culture-independent analyses. The aim of this review is to give a brief introduction of the microbial am- plicon HTS technology, to present a survey of recent aquatic food investigations via 16S rRNA amplicon HTS and to il- lustrate the potential of this approach for a valuable tool in the better characterization of aquatic foods and their pro- cessing and storage.