羚贝止咳糖浆止咳、平喘作用及其作用机制

Antitussive and Antiasthmatic Effect and Its Mechanism of Lingbei Zhike Syrup

目的:研究羚贝止咳糖浆止咳平喘效果及通过减轻肺组织病理性凋亡达到止咳平喘作用的实验研究。方法:将48只豚鼠随机分为模型组,阳性药组(川贝雪梨糖浆、氢溴酸右美沙芬组),羚贝止咳糖浆低、中、高剂量(1.27,2.55,5.1 g·kg^(-1))组,每组8只,通过豚鼠枸橼酸引咳实验考察羚贝止咳糖浆镇咳作用。将60只豚鼠随机分为模型组,阳性药组(川贝雪梨糖浆、氨茶碱组),羚贝止咳糖浆低、中、高剂量(1.27,2.55,5.1 g·kg^(-1))组,每组10只,通过豚鼠氯化乙酰胆碱、磷酸组胺混合引喘实验考察羚贝止咳糖浆平喘作用。取健康小鼠68只,利用烟叶和刨花各50 g早晚各熏蒸1次,1次30 min,连续熏蒸7 d,建立小鼠支气管炎模型,通过苏木素-伊红(hematoxylin and eosin,HE)染色法判定模型建立成功,小鼠支气管炎模型建立成功后通过免疫荧光组织化学法(immunofluorescent histochemistry,IHC)比较空白组、模型组、川贝雪梨糖浆组及羚贝止咳糖浆低、中、高剂量(2.14,4.28,8.56 g·kg^(-1))组,肺部组织病理性凋亡相关蛋白B细胞淋巴瘤/白血病-2(B celllymphoma/lewkmia-2,Bcl-2),自杀相关因子(factorassociatedsuicide,Fas)的积分吸光度,研究羚贝止咳糖浆对小鼠支气管炎模型肺部组织病理性凋亡相关蛋白Bcl-2,Fas蛋白表达量的影响。结果:羚贝止咳糖浆显著延长豚鼠咳嗽潜伏期,减少咳嗽次数。延长豚鼠引喘潜伏期。HE染色结果显示成功建立小鼠急性支气管炎模型,免疫荧光化学法结果显示,羚贝止咳糖浆可显著增加炎症肺组织Bcl-2蛋白,减少Fas蛋白的表达。结论:羚贝止咳糖浆通过调控肺部组织凋亡相关蛋白Bcl-2,Fas抑制肺部组织病理性凋亡,帮助恢复组织正常结构减轻肺组织炎症发挥止咳平喘作用。

Objective： To study the antitussive and antiasthmatic effect of Lingbei Zhike syrup and conduct the experimental study on reducing lung tissue＇s pathological apoptosis to reach the antiasthmatic effect.Method： The 48 guinea pigs were randomly divided into model group,positive control group（ Chuanbei Xueli syrup,Dextromethorphan Hydrobromide group）,Lingbei Zhike syrup low,medium and high dose groups（ 1. 27,2. 55,5. 1 g·kg^-1）,n = 8 in each group. Citric acid-induced cough experiment in guinea pig was used to investigate the antitussive effect of Lingbei Zhike syrup. The 60 guinea pigs were randomly divided into model control group,positive control group（ Chuanbei Xueli syrup,aminophylline group）,Lingbei Zhike syrup low,medium and high dose groups（ 1. 27,2. 55,5. 1 g·kg^-1）,n = 10 in each group. Acetylcholine-histamine phosphate-induced asthma experiment was used to investigate the antiasthmatic effect of Lingbei Zhike syrup. In 68 healthy mice,50 g tobacco leaves and wood shavings were fumigated once respectively in the morning and evening,30 min/time for continuous 7 days to establish bronchitis models. Hematoxylin and eosin（ HE） staining was used to determine whether the models were successfully established. After successful modeling,immunofluorescence histochemistry（ IHC） method was used to compare the blank control group,model control group,Chuanbei Xueli syrup group,and Lingbei Zhike syrup low,middle and high dose groups（ 2. 14,4. 28,8. 56 g·kg^-1） in lung tissue pathological apoptosis related protein B cell lymphoma/leukemia-2（ Bcl-2） and integral optical density value of factorassociatedsuicide（ Fas）,so as to investigate the effects of Lingbei Zhike syrup on apoptosis related protein Bcl-2 and Fas protein expression levels in lung tissues of bronchitis models. Result： Lingbei Zhike syrup could significantly prolong cough incubation period of guinea pigs and decrease the frequency of cough. HE staining showed that the mouse models of acute bronchitis were successfully established. Immunohistochemistry results showed that Lingbei Zhike syrup could significantly increase the expression level of Bcl-2 protein in lung tissues with inflammation and reduce the expression of Fas protein. Conclusion： Lingbei Zhike syrup could inhibit lung tissue＇s pathological apoptosis through regulating the expression of apoptosis-related proteins Bcl-2 and Fas,thus helping to restore the normal tissue structure and reduce lung tissue inflammation in playing antiasthmatic effects.