摘要
建立了高效液相色谱-波长切换法同时测定清宫长春胶囊中的尿囊素(1)、去氢土莫酸(2)、土莫酸(3)、茯苓酸(4)、梓醇(5)、吉奥诺苷B_1(6)、马替诺皂苷(7)、人参皂苷Rg_1(8)和人参皂苷Rb_1(9)。采用Agilent TC-C_(18)色谱柱;以乙腈∶0.2%磷酸为流动相,线性梯度洗脱;切换波长检测:224 nm(0→19 min,1)、210 nm(19→31 min,2~5)、330 nm(31→37 min,6、7)、203 nm(37→55 min,8、9)。1~9分别在8.0~160.0、2.0~40.0、2.5~50.0、2.0~40.0、5.0~100.0、2.5~50.0、2.0~40.0、15.0~300.0和8.5~170.0μg/ml范围内线性关系良好;平均回收率分别为98.82%、100.03%、97.92%、98.09%、97.64%、98.14%、96.91%、97.88%和98.39%,RSD<2%。
An HPLC-wavelength switching method was established for the determination of allantoin (I), dehydrotumulosic acid (2), tmnulosic acid (3), pachymic acid (4), catalpol (5), jionoside B1 (6), martinoside (7), ginsenoside Rg1 (8) and ginsenoside Rb1 (9) in Qinggong Changchun Jiaonang. An Agilent TC-C18 column was used, with the mobile phase of acetonitrile : 0.2 % phosphoric acid by gradient elution, at the detection wavelength of 224 nm (0-19 rain, 1), 210 nm (19-31 min, 2-5), 330 nm (31→37 min, 6 and 7) and 203 nm (37→55 min, 8and 9). Their calibration curves were linear in the ranges of 8.0-160.0, 2.0--40.0, 2.5--50.0, 2.0--40.0, 5.0--100.0, 2.5--50.0, 2.0 40.0, 15.0--300.0 and 8.5--170.0 μg/ml, respectively. Their average recoveries were 98.82%, 100.03%, 97.92%, 98.09%, 97.64%, 98.14%, 96.91%, 97.88% and 98.39%, with RSDs less than 2%. This method can be used for the quality analysis of Qinggong Changchun .Jiaonang.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2017年第11期1646-1649,共4页
Chinese Journal of Pharmaceuticals
关键词
清宫长春胶囊
高效液相色谱
波长切换
含量测定
Qinggong Changchun Jiaonang
HPLC
wavelength switching
content determination