液相色谱-串联质谱联用法检测人体类固醇激素的研究进展

Progress of liquid chromatography tandem mass spectrometry for the analysis of human steroid hormones

类固醇激素是胆固醇经一系列酶催化产生的脂溶性小分子，具有维持新陈代谢、调节性功能等重要作用，定量检测类固醇激素对许多内分泌疾病的诊断和治疗有重要意义。但由于类固醇激素涉及分子种类多、结构类似、体内含量低，定量检测分析存在一定的难度。目前，临床上多用免疫分析法检测类固醇激素，但一个免疫反应只能检测一种类固醇激素，很多激素没有特异的免疫反应可以用来检测，同时检测灵敏度和特异性也不都令人满意。液相色谱-串质谱（LC-MS/MS）分析法具有高灵敏度和高特异性的特定，并且可以一次进样同时定量检测多种类固醇激素，因而成为国内外临床研究的热点。

Steroid hormones are fat soluble molecules produced by cholesterol through a series of enzymatic catalysis. These low-level steroid hormones in body fluids generally play an important role in the regulation of metabolism and sexual function. Therefore, accurate quantification of these hormones is crucial for both diagnosis and treatment of endocrine disorder disease. At present, automated immunoassays are commonly used for the measurement of steroid hormone. However, only one analyte can be detected in one assay. More importantly, a lot of steroid hormones still cannot be detected by immunoassays due to the limitation of specific antibody. Liquid chromatography tandem mass spectrometry （LC-MS/MS） has high sensitivity and high specificity, and can simultaneously detect a variety of steroid hormones in one injection. LC-MS/MS has become a hot research topic in clinical research.（Chin J Lab Med, 2017, 40： 816-822）