间接免疫荧光法检测肺炎支原体IgM抗体对儿童肺炎支原体肺炎的临床诊断价值

Clinical Values of Mycoplasma Pneumoniae Pneumonia Antibody IgM Detecting by Indirect Immunofluorescence Method in the Diagnosis of Children with Mycoplasma Pneumoniae Pneumonia

目的:探讨间接免疫荧光法检测肺炎支原体IgM抗体对儿童肺炎支原体肺炎的临床诊断价值。方法:用间接免疫荧光法检测肺炎支原体肺炎患儿253例,健康人群正常对照组50例的肺炎支原体IgM抗体,比较其灵敏度,特异性。结果:肺炎支原体肺炎患儿组的肺炎支原体IgM抗体(MP-IgM)阳性比率高于正常对照组,差异具有统计学意义(x^2=38.07,P<0.05),用间接免疫荧光法检测肺炎支原体肺炎患儿的MP-IgM阳性率为65.21%,正常对照组的阳性率为18.00%;间接免疫荧光法诊断肺炎支原体肺炎的灵敏度为65.21%,特异性为82.00%。结论:间接免疫荧光法检测儿童血清中肺炎支原体IgM抗体有利于呼吸道感染患者的早期诊断,可为临床的诊疗提供重要的参考依据。

Objective：To explore and study Indirect immu- nofluorescence assay in Mycoplasma pneumonia antibody IgM （MP-IgM）and its clinical values in the diagnosis of children with Mycoplasma pneumoniae pneumonia. Methods： 253 cas- es of children with Mycoptasma pneumoniae pneumonia and 50 cases of normal controls healthy people were selected into this study, to compare their sensitivity and specigicity. Results： The positive rate of MP-IgM in children with Mycoplasma pneumoniae pneumonia were significantly higher than that of normal controls （x2=38.07, P 〈 0.05）. Wilh the method of Indi- rect immunofluoreseence assay in Mycoplasma pneumonia anti- body IgM,the positive rate of the group of children with Myco- plasma pneumonia is 65.21%,the other one is 18.00%.The sen- sitivity of 1FA for the diagnosis of Mycoplasma pneumoniae pneumonia was 65.21% and its specigicity was 82.00%. Con- clusion：Indirect immunofluorescence（IFA） for rapid detection of serum IgM antibodies can provide the important reference frame for clinical diagnosis and treatment, MP IgM test is helpful for the early diagnosis with respiratory tract infection.