维生素A对C_(57)BL小鼠黑色素瘤肺转移的影响及其机理研究

EFFECT OF VITAMIN A ON THE LUNG METASTASIS IN C_(57)BL MOUSE MELANOMA

本研究从免疫学角度观察了维生素A(V-A)对肺泡巨噬细胞(Mφ)功能的影响和体内抗肿瘤血道肺转移的作用。实验结果显示,V-A处理后小鼠肺泡Mφ细胞毒活性及其细胞表面补体C_3b受体功能均有所增强,小鼠肿瘤血道肺转移减少,且两者之间有较好的相关性。

Effects of vitamin A on antimetastatic activity of alveolar M 0 were studied. The results were as follows: 1 . SD rats recieved vitamin A were killed and their alveolar M0 were harvested by lavage and cocultured with prelabelled B16 Melanoma cells for 48h in proportion of 20 : 1 , the average precentage of cytotoxity were 34.28%; 2 . Zymosan-C3b rosette formation in SD rats treated with vitamin A was significantly increased. The average rosette forming rate was 43.00%. It was markdly higher than those of controls ( P< 0.01). 3. In vitamin A-treated C57BL mice the formantion of pulmonary metastasis were significantly inhibited . The inhibitory rate was 91.40%. In this experiment, there is a better correlation between the inhibition of pulmonary metastasis in vivo and increment of cytotoxic activity and C3b receptor of alveolar M0 in vitro.