声辐射力脉冲弹性成像技术对大鼠肝脏脂肪变性的诊断研究

Evaluation of rat liver steatosis by acoustic radiation force impulse elastography

研究目的是利用声辐射力脉冲弹性成像技术(Acoustic Radiation Force Impulse,ARFI)评估大鼠肝脏的脂肪变性程度。实验通过对大鼠喂养不同时间高脂食物从而诱导成肝脏脂肪变性不同阶段,进而利用ARFI技术离体测量大鼠肝脏的剪切波速度值(Shear Wave Velocity,SWV)。研究结果显示,对照组[(2.25±0.52)m/s)]与脂肪变性组[(2.83±0.37)m/s]之间存在着显著性差异(P<0.05),而脂肪变性不同阶段之间差异却不明显。早期炎症等级与SWV值之间没有显著性相关。因此,ARFI弹性成像技术可以有效地区分大鼠肝脏正常组和脂肪变性组,但是对不同脂肪变性程度之间无法做出有效区分。同时,轻微的炎症活动并不影响ARFI测量。

The goal of this study is to evaluate the utility of acoustic radiation force impulse ARFI elastography for determining the severity grade of rat liver steatosis. Steatosis is induced in the rat livers by gavage feeding of a high fat emulsion. Liver mechanics are measured ex vivo using shear wave velocity SWV induced by acoustic radiation force. The results show that there is a significant difference between the normal group 2.25±0.52 m/s and steatosis group 2.83±0.37 m/s P 0.05. However, there are no significant differences in SWV measurements between the steatosis grades. SWV values do not correlate with the early grade of inflammation. In conclusion, ARFI elastography is a promising method for differentiating normal rat liver from the rat liver with steatosis, but it cannot reliably predict the grade of steatosis in rat livers. The early grade of inflammation activity does not significantly affect the SWV measurements.