响应面优化超临界法萃取绿豆皮黄酮工艺

Supercritical Extraction of Flavonoids from Mung Bean Skin by Response Surface Methodology

采用超临界CO2技术萃取绿豆皮中黄酮类物质,通过单因素和响应面试验对超临界萃取绿豆皮黄酮工艺参数进行优化,结果表明:当萃取压力31 MPa、萃取温度47℃、萃取时间3.58 h、原料/夹带剂=1∶0.62(g/m L)时,绿豆皮黄酮的萃取率最高为87.64%。影响黄酮萃取率由强到弱的因素依次为萃取温度>萃取压力>萃取时间>原料/夹带剂。

Supercritical CO2 extraction was used to extract flavonoids from mung bean hulls. The optimum con-ditions were as follows： extraction pressure 31 MPa, extraction temperature 47 ℃ , extraction time 3.58 h and the ratio of raw material / entrainer = 1 ： 0.62 （g/mL）. Under these conditions, the extraction rate of flavonoids was 87.64 %. The results of analysis of variance also showed that the factors influencing flavonoid extraction rate from strong to weak were extraction temperature〉extraction pressure〉extraction time〉raw material / entrainer.