硫化铜萃取黄酮化合物的性能研究

Extraction abilities of copper sulfide micro-flowers for flavonoids

通过简单的水热反应合成了微花状的CuS,研究了其萃取黄酮化合物的性能.扫描电镜与粉末X射线分别表征了产物的形貌与晶相组成.将制备的微花状CuS填充于固相萃取(SPE)小柱中,以4种黄酮化合物为目标分析物,结合高效液相色谱(HPLC)考察了其萃取性能.对上样、洗脱等萃取参数进行了考察,在最优条件下,建立了CuS富集检测黄酮化合物的分析方法.结果表明,该方法具有较宽的线性范围(1~100μg·L^(-1)),较低的检测限(0.25μg·L^(-1)),单柱重复性及柱间重现性的相对标准偏差分别低于9.6%和11.4%.将该技术用于2种实际样品中黄酮化合物的分析检测,获得了较为满意的结果,证实了所制备的微花状CuS是一种性能优异的黄酮化合物萃取材料.

Copper sulfide micro-flowers are prepared by a simple hydrothermal method and their extraction abilities for flavonoids are studied. The product morphology and phase composition are characterized byscanning electron microscopy（SEM） and powder X-ray diffraction（XRD）. Coupled to a high performance liquid chromatography with a UV detector, the copper sulfide micro-flowers are packeextraction micro-column and their extraction abilities are accessed by four model flavonoid compounds. The parameters of sample loading and elution are investigated respectively, and the analytical methodbased on copper sulfide for flavonoids is established under the optimized conditions. The results showedthat the method owns wide linearities from 1 to 100 ＋g ？ L_1 and low limitsfor flavonoids, and the relative standard deviations for single-column repeatability and column-to-column reproducibility are less than 9. 6 % and 11. 4%. The method analyzes two real samples and satisfactoryresults are obtained. All the experimental results proved that the prepared copper sulfide micro-flowersare a good solid-phase extraction material for flavonoids.