基于Au@Ag/PPy复合纳米材料固载葡萄糖氧化酶的葡萄糖电化学传感器的构建

Construction of glucose electrochemical sensor based on composites of Au@Ag/PPy with immobilized glucose oxidase

制备了一种基于Au@Ag/PPy复合纳米材料固载葡萄糖氧化酶(GOD)的葡萄糖电化学传感器,并将其用于葡萄糖的检测.利用循环伏安法和差示脉冲法对电化学传感器的电化学行为进行研究,探讨了扫速、溶液pH对传感器峰电流的影响.结果表明,制备的电化学传感器对葡萄糖具有良好的电化学响应,在pH=7.0的条件下,电流响应信号与葡萄糖的浓度在2.3×10^(-7)~1.3×10^(-4)mol·L^(-1)范围内呈现良好的线性关系,检测限达到7.7×10^(-8)mol·L^(-1)(S/N=3).利用制备的葡萄糖电化学传感器对人血清中葡萄糖浓度进行检测,回收率达到98.0%~103.0%.

In this work, a novel electrochemical sensor for glucose based on composites of A u @ Ag/P Py with immobilized glucose oxidase（GOD） is successfully constructed, and then used for the determinationof glucose. The electrochemical behavior of glucose based on composites is studied by cyclic voltammetry and differential pulse technique. Additionally, the influence of scan rate and pH value on the peak currentof sensor is discussed. The results showed that this sensor exhibits remarkable current signal in theprocess of glucose detection, and good linear relationship between the current signal and concentration ofglucose in the range from 2. 3 × 10-7 ～ 1. 3 × 10-4 mol · L-1 in pH = 7. 0, as well 7. 7 × 10-8 mo/ L-1 （S/N = 3）. In addition,the actual sample is detected and the recovery rate is 98. 0%-103. 0%.