摘要
建立了一种高效液相色谱测定水样中4种苯系物(苯、甲苯、乙苯、对二甲苯)的方法。分析柱为Diamonsil5 μ mC18,250×4.6mm,检测波长为259nm,流动相比例V(乙腈):V(水)=75:25,流量大小为1.000mL/min,色谱柱温度为35℃。方法的线性范围为0.0100~10.0mg/L,相关系数大于0.9997,4种苯系物的检出限(S/N=3)为1.9μg/L^3.2μg/L,方法的重复性良好,峰面积的相对标准偏差(n=7)小于4.6%。样品中未检出,采用加标浓度为0.100mg/L和1.00mg/L进行方法验证,实际水样的加标回收率为82.3%~97.6%,表明该方法可有效用于水样中苯系物的灵敏、准确分析。
A method for the simultaneous determination of four benzenes,(benzene, toluene, ethylbenzene, and p-xylene) in water samples was established using high performance liquid chromatography. The analytical column was performed on a Diamonsil C18(5μm×4.6 mm×250 mm), the quantitation analysis of four benzenes were achieved by UV detection at 259 nm, the mobile phase used was acetonitrile/water(75:253, v/v), the flow rate of the mobile phase was 1.000 mL/min, and the column temperature was kept on 35 °C. The linear ranges of four benzenes were in the range of 0.0100 mg/L-10.0 mg/L with correlation coefficients more than 0.9997. The method detection limits(S/N=3) of four benzenes were between 1.9 μg/L and 3.2 μg/L. The relative standard deviations(n=7) were less than 4.6%. The spiked recoveries at two matrix spiked concentrations were in the range of 82.3%~97.6%. The results showed that the developed method was sensitive and accurate,and was suitable for the analysis of benzenes in water sample.
作者
王兴益
陈兴舟
杨燕群
刘玉兰
李明刚
陈君雨
张仟春
WANG Xingyi CHEN Xingzhou YANG Yanqun LIU Yulan LI Minggang CHEN Junyu ZHANG Qianchun(Xingyi Normal University for Nationalities, Xingyi, Guizhou 562400, Chin)
出处
《兴义民族师范学院学报》
2017年第3期119-124,共6页
Journal of Minzu Normal University of Xingyi
基金
贵州省科学技术基金(黔科合LH字[2014]:No.7406)
黔西南州科技局项目(No.2016-1-32
No.2016-1-29)
关键词
苯系物
高效液相色谱
环境水样
Benzenes
High Performance Liquid Chromatography
Environmental Water Samples