大株红景天注射液对脓毒症急性肺损伤小鼠肺组织核因子-κB表达的影响

Effect of sofren injection on NF-κB expression in acute lung injury mice with sepsis

目的观察大株红景天注射液对脓毒症小鼠急性肺损伤的影响及可能机制。方法将72只雄性昆明小鼠随机分为4组(n=18):假手术组(Sham组)、急性肺损伤组(ALI组)、溶媒组(Vehicle组)、大株红景天注射液组(Sofren组)。ALI组、Vehicle组和Sofren组用盲肠结扎穿孔法制备脓毒症模型。Vehicle组和Sofren组分别于造模完成时立即腹腔注射溶媒或大株红景天注射液4 mL/kg,以后每12h一次重复给药。术后12 h、24 h及48 h取肺组织,行HE染色后光镜下观察肺组织病理损伤,Western blot法检测肺组织磷酸化IκBα(P-IκBα)、IκBα,磷酸化NF-κBp65(P-p65)的表达。结果与Sham组相比,ALI组及Vehicle组肺损伤加重,P-IκBα、P-IκBα/IκBα及P-p65表达明显增加(P<0.05);与ALI组相比,Sofren组肺损伤轻,P-IκBα、P-IκBα/IκBα及P-p65表达明显减少(P<0.05)。结论大株红景天注射液减轻脓毒症小鼠急性肺损伤,其机制可能与抑制P-p65表达有关。

Objective To observe the effect of sofren injection on septic acute lung injury in mice and its mechanism. Methods 72 male Kunming mice were randomly divided into 4 groups( n = 18) : the sham group,the ALI group,the vehicle group,and the sofren injection group. Sepsis model was established by cecal ligation and puncture( CLP) in the group ALI,vehicle and sofren. In the group vehicle and sofren,4 mL/kg vehicle or sofren injection were intraperitoneally injected respectively after operation,followed by administration q12 h. At 12,24 and 48 h after operation,the whole lung were removed to observe the lung pathological damage after hematoxylin-eosin staining,and to detect the expression of P-IκBα,IκBα and NF-KBp65( using Westen blot) in lung tissues. Results As compared with the sham group,the pathological damage of lung and the level of P-IκBα,P-IκBα/IκBα and PIκBα at 12,24 and 48 h after operation were significantly increased in the ALI group( P < 0. 05). Compared with the ALI group,the pathological damage of lung and the level of P-IκBα,P-IκBα/IκBα and P-IκBα at each time were significantly decreased in the sofren group( P < 0. 05). Conclusion Sofren injection inhibits the expression of Pp65,thus protects sepsis-induced ALI in mice.