基于PCR-反向点杂交法对江西地区分枝杆菌感染状况分析

Epidemiology of mycobacteria infection by PCR-reverse dot hybridization in Jiangxi area

目的了解江西地区分枝杆菌感染流行现状,为临床防治提供科学依据。方法运用PCR-反向点杂交法对345例可疑分枝杆菌感染患者不同标本进行分枝杆菌核酸检测。结果 345份标本中共检出68例阳性(阳性率为19.7%),其中结核分枝杆菌复合群(MTC)59例(占86.76%),非结核分枝杆菌(NTM)8例(占11.77%),混合感染(MTC/MIN)1例(1.47%);NTM感染中检测出5种,分别为偶发/猪分枝杆菌(MFO)、戈登分枝杆菌(MGO)、胞内分枝杆菌(MIN)、龟分枝杆菌脓肿亚肿(MAB)、蟾蜍分枝杆菌(MXE),其中以MIN为主,占5.88%;不同标本中分枝杆菌检出率不同,痰液、灌洗液、分泌物、胸水中分枝杆菌检出率分别为:31.5%、20.3%、16.0%、4.7%。结论江西地区分枝杆菌感染主要以MTC为主,且多为单一感染。

Objective To understand the prevalence of mycobacteria infection in Jiangxi region and to provide a scientific basis for clinical prevention and treatment. Methods The different samples of 345 patients who suspected with mycobacteria infection were tested by PCR-reverse dot hybridization. Results 68 cases were positive in 345 specimens,including 59 cases of mycobacteria tuberculous complex( MTC)( 86. 76%) and 8 cases of nontuberculous mycobacteria( NTM)( 11. 77%) and 1 case of mixed infection( MTC/MIN)( 1. 47%). There were5 kinds of NTM infection detected,namely MFO,MGO,MIN,MAB and MXE respectively,and MIN was priority to others in NTM,accounted for 5. 88%. The positive rate was various in different specimens,which sputum,lavage,secretions,pleural effusion positive rate was31. 5%,20. 3%,16. 0%,and 4. 7% respectively. Conclusion MTC and single infection are mainly types of mycobacterial infection in Jiangxi region.