非小细胞肺癌患者肺癌自身抗体检测的临床意义

Detection of auto-antibodies of lung cancer in non-small cell lung cancer patients

目的探讨血清7种肺癌自身抗体在非小细胞肺癌(NSCLC)患者中的水平及其临床意义。方法采用酶联免疫吸附法(ELISA)检测健康人、肺部良性疾病患者和NSCLC患者血清中7种肺癌自身抗体(p53、PGP、SOX2、GAGE7、GBU4-5、MAGEA1、CAGE)的表达量,并计算它们诊断肺癌的敏感性和特异性。结果NSCLC患者血清中7种肺癌自身抗体的表达水平显著高于健康人对照组和肺部良性疾病组,差异有统计学意义(P<0.05);肺癌自身抗体p53、PGP、GBU4-5和CAGE在Ⅲ期+Ⅳ期NSCLC中的表达水平显著高于Ⅰ期+Ⅱ期(P<0.05);血清7种肺癌自身抗体对NSCLC检测的敏感性为54.5%、特异性为94.3%;7种肺癌自身抗体对I期、II期NSCLC检测的敏感性为34.6%。结论血清肺癌自身抗体的检测对于NSCLC的辅助诊断和早期诊断有一定的价值。

Objective To evaluate the clinical significance for diagnosis of detection of 7 types serum autoantibodies in non-small cell lung cancer patients( NSCLC). Methods Enzyme linked immunosorbent assay( ELISA) method was used to examine the serum 7 types autoantibodies( p53,PGP,SOX2,GAGE7,GBU4-5,MAGEA1,CAGE) levels in NSCLC patients,healthy controls and patients with respiratory infection of the lung. The sensitivity and specificity of diagnosis for NSCLC were calculated. Results The level of 7 types auto-antibodies in patients with NSCLC was significantly higher than that of patients with respiratory infection of the lung and healthy controls( P < 0. 05). The level of auto-antibodies( p53,PGP,GBU4-5 and CAGE) was significantly higher at stageⅢ and Ⅳ of NSCLC than that of stage at Ⅰ and Ⅱ( P < 0. 05). The sensitivity and specificity of 7 types auto-antibodies in NSCLC were 54. 5% and 94. 3%. The sensitivity and specificity of 7 types auto-antibodies in NSCLC of stages I and II was 34. 6%. Conclusion The detection of auto-antibodies in serum has certain clinical significance in aid diagnosis and early diagnosis of NSCLC.