新型序贯应激法致焦虑样胃高敏感性大鼠功能性消化不良模型的建立

Establishment of an anxiety-like gastric hypersensitivity rat model offunctional dyspepsia induced by a new method of sequential stress

目的应用新型序贯应激法建立焦虑样胃高敏感性大鼠模型。方法将新生大鼠幼仔随机分为对照组与模型组,每组13只。出生后第2天起序贯采用胃刺激法、母婴分离法和束缚应激法制作焦虑样胃高敏感性模型。于第8周开始采用高架十字迷宫试验（EPM）、旷场试验（OF）、腹肌回缩试验（AWR）、肌电图试验（EMG）和胃排空试验（GET）等评价模型成功与否。结果 EPM和OF实验提示模型组大鼠较对照组表现出明显的焦虑样行为,AWR和EMG实验显示模型组大鼠对胃扩张刺激的敏感性亦显著升高（P〈0.05,P〈0.01）,但两组在体重变化和胃黏膜炎症反应方面差异无显著性,符合胃敏感性增高为主功能性消化不良的临床特点。结论应用新型序贯应激法可成功建立焦虑样胃高敏感性大鼠模型。

Objective To establish an anxiety-like gastric hypersensitivity（ GHS） rat model of functional dyspepsia induced by new sequential stress. Methods Twenty-six male 1-day-old Sprague-Dawley rat pups were randomly divided into control and model groups（ n = 13 in each group）. The model rats received sequential stress from postnatal day2： neonatal maternal separation（ NMS）,acute gastric irritation（ AGI） and restraint stress（ RS）. The control rats were reared freely with their mothers without undergoing the sequential stress. From postnatal 8^（th） week all rats started to receive elevated plus maze（ EPM）,open field test（ OF）,abdominal withdrawal reflex（ AWR） and electromyographic test（ EMG）. Results EPM and OF experiments depicted that the model rats showed obvious anxiety-like behavior（ P〈0. 05 or P〈0. 01）. AWR and EMG tests exhibited that the model rats had elevated gastric hypersensitivity to gastric distention（ P〈0. 05 or P〈0. 01）. Conclusions An anxiety-like GHS rat model of functional dyspepsia can be successfully established with our new method of sequential stress.