高效液相色谱法同时测定塑料和纸质包装材料中6种荧光增白剂

Simultaneous determination of six fluorescent whitening agents in plastic and paper packaging materials by high performance liquid chromatography

建立了同时测定塑料和纸质食品包装材料中6种脂溶性荧光增白剂(FWA 135、FWA 184、FWA 185、FWA199、FWA 378和FWA 393)的高效液相色谱方法。用三氯甲烷-乙腈(3∶7,v/v)混合溶液提取,经HLB小柱净化后用高效液相色谱-荧光检测法进行定性定量分析。采用Phenomenex C18色谱柱分离,以5 mmol/L的乙酸铵水溶液和乙腈为流动相,进行梯度洗脱。结果显示:FWA 393在15~1500μg/L范围内的线性关系良好,其余5种荧光增白剂在5~500μg/L范围内线性关系良好,相关系数均大于0.999;加标回收率为80.4%~125.0%;相对标准偏差(RSD,n=6)为1%~13%。应用该方法分析了市场销售的12个样品以验证方法的实用性。该方法前处理简单,回收率高,精密度好,适用于食品包装材料中6种荧光增白剂的检测。

A novel analytical method was developed for the simultaneous determination of six fluorescent whitening agents （FWAs： FWA 135,FWA 184,FWA 185,FWA 199,FWA 378 and FWA 393） in paper and plastic food packaging materials by high performance liquid chromatog-raphy with fluorescence detection （HPLC-FLD）. The sample was extracted with mixed solution of chloroform and acetonitrile （3 ： 7, v /v ） ,then cleaned up by HLB solid phase extraction col-umn. Qualitative and quantitative analyses were carried out by HPLC. The sample was separated on a Phenomenex C18 column using acetonitrile and 5 mmol/L ammonium acetate aqueous solution as mobile phases. The results indicated that the linear range of FWA393 was 15-1500 μg/L and the linear ranges of the other five FWAs were 5-500 μg/L with correlation coeffi-cients greater than 0.999. The recoveries in spiked samples were between 80. 4% and 125. 0% with RSDs （n = 6） of 1%-13%. Furthermore,this method was applied to analyze 12 samples in the market to verify the practicality of the method. The method showed the advantages of sim- plicity,high recovery and good precision,and is suitable for the detection of the six fluorescent whitening agents in food packaging materials.