黄芩苷对辐射诱导的小鼠急性肺损伤保护作用机制研究

Effect of Baicalin on Radiation-Induced Acute Lung Injury in Mice Through Antioxidant and Anti-Inflammatory Properties

目的探讨黄芩苷对辐射诱导小鼠的急性肺损伤保护作用。方法 X-线复制小鼠的急性肺损动物模型。将50只ICR小鼠随机分为空白对照组、X-线组、X-线组+地塞米松组(5mg/kg)、X-线组+黄芩苷组(20、40mg/kg)。观察各组肺组织病理学改变,测量肺湿/干质量比,支气管肺泡灌洗液中性粒细胞百分比、肺组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、炎症因子白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)含量及肺组织中p-NFκBp65、p-IκBα蛋白表达。结果黄芩苷组(20、40mg/kg)可有效减轻辐射所致肺组织病理学变化,能降低肺泡灌洗液中炎症细胞的数目,能显著降低肺湿/干质量比重,能提高SOD水平,并能降低MDA水平,降低p-NFκBP65和p-IκBα蛋白的表达。结论黄芩苷可减轻辐射所致急性肺组织损伤,对辐射诱导的急性肺损伤有保护作用。

OBJECTIVE The current study was designed to evaluate the protective effects of baicalin on radiation-induced acute lung injury in mice.METHODS 50 ICR mice were divided into five groups randomly,namely,control group,X-ray group,X-ray+dexamethasone group(5 mg/kg),X-ray+baicalin group(20,40 mg/kg),with 8 in each group.Pathological changes in the lung tissues were observed.The ratio of lung wet/dry weight was also recorded.Besides,the level of neutrophil in bronchoalveolar lavage fluid(BALF)was detected to evaluate inflammatory cell infiltration.Moreover,the expressions of MDA,IL-6,TNF-α,p-NFκBp65 and p-IκBα,as well as the activity of SOD,were examined in lung.RESULTS Baicalin(20,40 mg/kg)could effectively improve the radiation-induced lung pathological changes and reduce the number of inflammatory cells in BALF.The ratio of lung wet/dry weight was also decreased with the treatment of baicalin(20,40 mg/kg).In addition,the activity of SOD was recovered and the end product of lipid peroxidation MDA was reduced meanwhile.The expressions of p-NFκBp65 and p-IκBαwas extensively down-regulated with baicalin(20,40 mg/kg)preconditioning,taking the expressions of NFκBp65 and IκBαas inner controls.CONCLUSION Baicalin could alleviate radiation-induced acute lung injury due to its antioxidant and anti-inflammatory properties.