亚甲基四氢叶酸还原酶C677T基因多态性与H型高血压的关系

Correlation between MTHFR C677T gene polymorphism and H-type hypertension

目的探讨亚甲基四氢叶酸还原酶(MTHFR)C677T基因多态性与H型高血压的关系。方法 273例高血压患者,其中H型高血压(血浆Hcy≥10μmol/L)185例(观察组)、非H型高血压88例(对照组)。取两组空腹静脉血,用PCR-RFLP方法观察MTHFR C677T基因多态性。采用logistic回归分析MTHFR C677T基因型与H型高血压的关系。结果观察组、对照组MTHFR C677T基因型CC分别为31、35例,CT分别为104、45例,TT分别为50、8例,C等位基因分别为166、115例,T等位基因分别为204、61例,两组CC、TT基因型及C、T等位基因分布比较P均<0.01。logistic回归分析结果显示,MTHFR C677T基因型突变(OR=2.645,95%CI:1.737~4.025,P<0.01)是H型高血压的独立危险因素。结论 MTHFR C677T基因型TT是H型高血压发病的独立危险因素。

Objective To explore the relationship between the methylenetetrahydrofolate reductase （MTHFR） C677T gene polymorphism and H-type hypertension.Methods A total of 273 consecutive patients with hypertension,including185 cases of patients with H-type hypertension （observation group,Hcy≥10 μmol/L） and 88 cases of patients with nonH-type hypertension （control group）,were enrolled.We collected the fasting venous blood in the two groups,then,the MTHFR C677T gene polymorphism was observed by PCR-RFLP.The relationships between the genotypes of MTHFR C677T and H-type hypertension were analyzed by Logistic regression.Results There were 31 and 35 cases of MTHFR C677T CC genotype in the observation group and control group,and the number of CT genotype was 104 and 45,respectively; the number of TT genotype was 50 and 8,respectively; the number of C allele of the two groups was 166 and 115,respectively; the number of T allele of the two groups was 204 and 61,respectively; Significant difference was found in the distribution of the allelic frequencies of T and C,and CC and TT genotypes between the two groups （P〈0.01）.Logistic regression analysis showed that MTHFR C677T genotype mutation （OR = 2.645,95% CI： 1.737-4.025,P〈0.01） was an independent risk factor for H-type hypertension.Conclusion MTHFR C677T genotype TT is an independent risk factor for H-type hypertension.