MTA2在舌癌组织中的表达及其意义

Expression and significance of metastasis-associated protein 2 in tongue carcinoma

目的比较舌癌患者和正常人中转移相关蛋白2(metastasis associated protein 2,MTA2)的mRNA和蛋白表达水平,并探讨其差异的临床意义。方法选取经病理确诊为舌鳞癌患者95例,同时选取癌旁正常舌黏膜25例;同期另各取20例舌癌组织与20例癌旁正常上皮组织,采用免疫组化方法检测95例确诊为舌癌组织及25例相应癌旁正常舌黏膜上皮组织中MTA2的表达;采用RT-PCR和Western Blot分别检测20例舌癌组织与癌旁正常上皮组织中mRNA和蛋白的表达。分析MTA2在舌癌组织中的表达与患者的性别、年龄及癌组织分化程度的关系。结果 MTA2的mRNA和蛋白在舌癌组织中的表达明显高于癌旁组织(P<0.05)。MTA2在舌癌组织中的表达与患者性别、年龄无显著相关(P>0.05),而与癌组织的分化程度呈负相关(P<0.05)。结论 MTA2在舌癌组织中的表达高于癌旁组织,MTA2的表达与癌组织的分化程度呈负相关。

Objective To observe the expressions of mRNA and protein of metastasis-associated protein 2（MTA2） in tongue carcinoma and explore its clinical significance.Methods Protein expression of MTA2 in 95 specimens of tongue carcinoma and 25 of para-tumor normal mucosa epithelial tissue was detected by immun ohistochemical method.The expressions of MTA2 mRNA and MTA2 protein in 20 specimens of tongue carcinoma and 20 of para-tumor normal tissue were measured by real-time polymerase chain reaction（RT-PCR） and Western Blot respectively.The relationships between the expressions and patients＇ gender,age and the degree of differentiation of carcinoma tissue were analyzed.Results（1） The expressions MTA2 mRNA and MTA2 protein in the tongue carcinoma tissues were significantly increased compared with the para-tumor normal tissues（both P〈0.05）.（2） There were no significant relationships between the expressions and patients＇ gender and age（all P〉0.05） while the expressions were negatively correlated with the differentiation degree of carcinoma（both P〈0.05）.Conclusions MTA2 expression in tongue carcinoma tissue is higher than that in the para-tumor normal tissue.MTA2 expression is negatively correlated with the differentiation degree of carcinoma.