自上而下法评定生化测量不确定度在国际旅行卫生保健中心实验室中的应用

Application of evaluating biochemical measurement uncertainty using Top-Down method in international travel health care center laboratory

目的探讨Top-Down法评定生化测量不确定度在卫生检疫国际旅行卫生保健中心实验室中的应用。方法收集实验室室内质控(IQC)数据和实验室参加临床检验中心室间质评(EQA)结果,采用Top-Down法对血清丙氨酸氨基转移酶(ALT)等生化六项检测,进行测量不确定度评定。结果深圳迈瑞BS-300全自动生化分析仪检测ALT、天门冬氨酸氨基转移酶(AST)、γ-谷氨酰基转移酶(GGT)、葡萄糖(GLU)、甘油三酯(TG)和总胆固醇(TC)生化六项的相对合成标准测量不确定度[uc(%)]分别为7.94%、7.46%、7.67%、6.08%、5.80%和4.28%,均小于CLIA'88设置的目标不确定度。结论 Top-Down法评定生化测量不确定度适合在卫生检疫国际旅行卫生保健中心实验室中应用。

Objective To discuss application of evaluating biochemical measurement uncertainty using Top-Down method in health quarantine international travel health care center laboratory. Methods Collecting laboratory internal quality control（ IQC） data and external quality assessment（ EQA） results participated from clinical inspection center,to evaluate six biochemical measurement uncertainties such as serum alanine aminotransferase（ ALT） using Top-Down method. Results The relative synthesis standard measurement uncertainties[uc（ %） ] of ALT,aspartate aminotransferase（ AST）,gamma pancreatic acyl transferase（ GGT）,glucose（ GLU）,triglyceride（ TG） and total cholesterol（ TC） tested on Shenzhen Mindray BS-300 fully automatic biochemical analyzer were 7. 94%,7. 46%,7. 67%,6. 08%,5. 80% and 4. 28%,respectively,less than the goal uncertainties set by CLIA＇88. Conclusion Top-Down method to evaluate biochemical measurement uncertainty is suitable for the application in health quarantine international travel health care center laboratory.