microRNA-378i抑制横纹肌肉瘤细胞增殖和迁移

microRNA-378i Inhibits the Proliferation and Migration of Rhabdomyosarcoma Cells

该文主要研究了microRNA-378i(miR-378i)在人横纹肌肉瘤细胞中的表达水平及其对细胞增殖和迁移能力的影响。通过q RT-PCR(quantitative RT-PCR)法检测在横纹肌组织和横纹肌肉瘤细胞中miR-378i成熟体的水平。采用阳离子脂质体介导的方法将miR-378i成熟体转染入横纹肌肉瘤细胞,通过MTS法检测细胞增殖能力、细胞克隆形成实验检测细胞增殖和生长能力、流式细胞技术检测细胞周期,以及应用x CELLigence细胞功能分析仪检测细胞迁移能力。Western blot方法检测miR-378i对靶基因蛋白质水平的调控。结果显示,在横纹肌肉瘤细胞中,miR-378i成熟体的水平较正常横纹肌组织显著下调。恢复miR-378i的表达水平能显著抑制横纹肌肉瘤细胞的增殖、细胞克隆形成和迁移能力,并诱导细胞发生G1或G2期阻滞。Western blot结果显示,miR-378i能够下调IGF1Rβ的蛋白质水平。综上所述,miR-378i能够显著抑制横纹肌肉瘤细胞的增殖和迁移。

This study investigated the level of micro RNA-378i（miR-378 i） in rhabdomyosarcoma cells and striated muscle specimen and the influence of proliferation and migration caused by miR-378i in rhabdomyosarcoma cells. Quantitative RT-PCR（qRT-PCR） was performed to determine the level of miR-378 i in human rhabdomyosarcoma cells and striated muscle specimens. Lipofectimine was used to transfect miR-378 i mimics into rhabdomyosarcoma cells to upregulate the expression level of miR-378i. MTS, clone formation experiments, x CELLigence analysis and flow cytometry were performed to detect the proliferation, growth, migration and cell cycle of rhabdomyosarcoma cells, respectively. Western blot was used to identify the protein level of IGF1 Rβ. The results showed that the level of miR-378i in rhabdomyosarcoma cells was significantly decreased compared with striated muscle specimens. Recovery expression level of miR-378i inhibited cell proliferation and migration, decreased the number of clones, and induced G1 or G2 phase arrest in rhabdomyosarcoma cells. It was also found that miR-378 i could deregulate protein level of IGF1 Rβ. In conclusion, miR-378 i inhibited the proliferation and migration of rhabdomyosarcoma cells.