高丽红参进口药材标准含量测定方法的改进

Modication of the Content Determination Method on Korean Red Ginseng

目的通过改进供试品溶液的制备过程,采用紫外检测器建立测定高丽红参含量的高效液相色谱(HPLC)法,以改进进口药材标准中的含量测定方法。方法色谱条件为Agilent poroshell C_(18)柱(4.6 mm×75 mm,5μm);以乙腈-水为流动相进行梯度洗脱,流速为0.7 mL·min^(-1),检测波长为203 nm,柱温为25℃。供试品溶液采用水饱和正丁醇加热回流提取进行制备。结果改进方法的人参皂苷Rg_1在0.920 9~9.208 5μg、人参皂苷Re在0.928 0~9.279 8μg、人参皂苷Rb_1在0.955 2~9.551 8μg范围内与峰面积呈良好的线性关系;人参皂苷Rg_1、人参皂苷Re、人参皂苷Rb_1平均加样回收率分别为100.9%,102.5%,101.7%(n=6)。本方法结果比原标准方法的结果略高,相对偏差均小于5%。结论改进后的方法操作简单准确,重现性好,能提高检测效率。

OBJECTIVE To modify the quantitative determination method for imported Korean red ginseng by HPLC. METHODS HPLC was performed on the column of Agilent poroshell C18（4.6 mm×75 mm,5μm） with mobile phase of acetonitrile- water （gradient elution） at the flow rate of 0.7 mL·min^-1. The detection wavelength was 203 nm, and the column temperature was 25 ℃. Test solution was prepared by heating reflux with water saturated n-butanol. RESULTS The linear range of ginsenoside Rgl, ginsenoside Re and ginsenoside Rb1 were 0.920 9-9.208 5 μg, 0.928 0-9.279 8 μg and 0.955 2-9.551 8 μg, and the average recovery rates were 100.9%, 102.5%, 101.7%, respectively. The results obtained by the modified method were similar to those measured by the original method, and the relative deviation was less than 5% . CONCLUSION The improved method has the advantages of simple operation, high accuracy and good repeatability, and can be used to improve efficiency of detection.