马铃薯青薯九号甜菜碱醛脱氢酶基因(StBADH)克隆及进化分析

Isolation and Evolutional Analysis of StBADH in Qingshu 9(Solanum tuberosum)

本研究利用同源克隆,从马铃薯青薯9号中克隆到2个甜菜碱醛脱氢酶基因,分别命名为St BADH-504和St BADH-505。St BADH-504为1 631 bp,CDS为1 515 bp,编码504个氨基酸蛋白,编码蛋白亚细胞定位于线粒体;St BADH-505为1 709 bp,CDS为1 518 bp,编码505个氨基酸蛋白,编码蛋白亚细胞定位于叶绿体或过氧化物酶体,二个蛋白都有醛脱氢酶高度保守的十肽(VSLELGGKSP)结构。进化分析表明,St BADH-504和St BADH-505蛋白与茄科植物中BADH蛋白聚为一类,但二者分属不同分支。盐胁迫表达特征分析表明,马铃薯叶片中St BADH-504表达受盐胁迫的诱导,而St BADH-505为组成型表达,不受盐胁迫诱导。本研究有助于了解马铃薯耐盐机理,为耐盐马铃薯资源创制提供理论参考。

Two St BADH genes named St BADH-504 and St BADH-505 were isolated from potato cultivar Qingshu9 by Homology-based cloning in this study. St BADH-504 was 1 631 bp, containing 1 515 bp open reading frame（ORF） which encoded a protein of 504 amino acid residues. Subcellular localization of encoded protein was in the Mitochondria. St BADH-505 was 1 709 bp, containing 1 518 bp open reading frame（ORF） which encoded a protein of 505 amino acid residues. Subcellular localization of encoded protein was in the Chloroplasts and Peroxidase. A highly conserved decapeptide sequence（VSLELGGKSP） was found in both two deduced proteins.According to evolutional analysis, St BADH-504 and St BADH-505 were divided into solanaceae BADH proteins,but each belonged to different subgroups. Expression of analysis under salt stress indicated that St BADH-504 in leaf was induced by salt stress, while St BADH-505 was of constitutive expression and was not induced by salt stress. The results will be useful for understanding the salt-tolerance mechanism in potato and providing reliablebase for salt-tolerant potato resources breeding.