金鱼草丙二烯环化酶基因AmAOC的克隆及表达分析

Cloning and Expression Analysis of Allene Oxide Cyelase Gene AmAOC from Snapdragon

作为植物信号分子的茉莉酸(JA)参与植物的发育与代谢过程,丙二烯环化酶(AOC)是JA合成途径的关键酶。在本研究中,利用同源克隆结合RACE技术从金鱼草中克隆AOC基因,命名为Am AOC。Am AOC基因开放阅读框长762 bp,由254个氨基酸残基组成,该基因包含一个Alleneoxcyc结构域,有两个内含子和三个外显子。同源比对分析结果表明Am AOC与其他植物的AOC有较高的同源性。Am AOC基因在不同组织中的表达分析发现金鱼草花中的Am AOC相对表达量较高,不同花期金鱼草RT-PCR表明,花蕾期的Am AOC相对表达量最高,盛花期的不同花器官荧光定量PCR分析表明,金鱼草花筒和花萼中Am AOC相对表达量较高。

As a signaling molecule, jasmonic acid（JA） plays an important role in the development and metabolism of plants, and allene oxide cyclase（AOC） is a key enzyme in its biosynthesis. In the present study,the full length c DNA sequence of AOC gene named as Am AOC in Snapdragon（Antirrhinum majus L.） was cloned via homology cloning and RACE technique.The ORF of Am AOC gene was 762 bp encoding a protein composed of 254 amino acids.Am AOC protein contains a conserved Alleneoxcyc domain, and had 3 exons truncated with 2 introns.Homology analysis showed that Am AOC was highly homologous to AOC of other plants. The expression analysis of Am AOC gene in different tissues revealed that high transcript levels occurred in the flower, and the highest expression levels were found in the tubes and sepals. Among the different flower stages, the highest expression level of Am AOC appeared at the blooming stage.