蜈蚣珊瑚愈伤诱导及其分化正交试验设计

Callus Induction and Differentiation in Pedianthus tithymaloides cv. Nana by Orthogonal Experimental Design

本研究以蜈蚣珊瑚茎段为外植体,采用正交试验设计,进行愈伤组织诱导及分化研究,并建立植株再生体系。研究表明:愈伤组织诱导的最佳外植体为带叶茎段,最佳灭菌时间为0.1%的升汞6 min;愈伤组织诱导的最佳培养基为MS+4.0 mg/L 6-BA+0.2 mg/L IBA+0.1 mg/L NAA;不定芽增殖最佳培养基为MS+3.0 mg/L 6-BA+0.3 mg/LNAA,最佳继代次数为4次;愈伤组织不定芽诱导最佳碳源为蔗糖;生根培养最佳培养基为1/2 MS+0.4 mg/L IBA。本研究通过对蜈蚣珊瑚进行离体培养,初步获得组织培养各阶段最佳培养基配方,从而实现蜈蚣珊瑚离体快繁的目的,为蜈蚣珊瑚工厂化育苗提供理论基础及技术指导。

In this study, we took stems of Pedianthus tithymaloides cv.Nana as explants, to study the best medium for callus induction and differentiation by orthogonal experimental design and establish the plant regeneration system. Results showed that the best explants for callus induction were the stems with leaves, the best sterilization time was 0.1% mercuric chloride for 6 minutes. The optimal medium of callus induction was MS＋4.0 mg/L6-BA＋0.2 mg/L IBA＋0.1 mg/L NAA. The best medium for adventitious bud proliferation was MS＋3.0 mg/L6-BA＋0.3 mg/L NAA, and the best subculture times were 4. the best carbon source for adventitious bud induced by callus was sucrose. The most suitable rooting medium was 1/2 MS＋0.4 mg/L IBA. In this study, the explants of Pedianthus tithymaloides cv.Nana were cultured in vitro, and the optimum culture medium formula of each stage of tissue culture was obtained preliminarily, The purpose of this study was to achieve the rapid propagation, and to provide theoretical and technical guidance for the industrialization of plantlets of Pedianthus tithymaloides cv. Nana.