不同品种甜樱桃花色苷的积累及PacMYBA表达分析

Expression Analysis of PacMYBA Gene and Accumulation of Anthocyanins in Prunus avium

针对果实成熟时表现不同颜色的甜樱桃品种(黄色品种‘佐藤锦’,红色品种‘拉宾斯’),采用高效液相法测定分析其果实生长中花色苷主要成分及含量,采用RT-PCR克隆‘佐藤锦’的一个MYB基因,同时利用荧光定量PCR分析Pac MYBA基因在两个品种甜樱桃果实生长过程中的表达情况。结果表明,‘拉宾斯’果实转色后花色苷的积累上升趋势明显,成熟后花色苷含量高且组分由单一的一种逐渐丰富为3种;‘佐藤锦’果实转色后花色苷含量有所增加但增加幅度不大,且花色苷组分单一;二者在转色后Pac MYBA的相对表达上升趋势明显,成熟时‘拉宾斯’Pac MYBA的相对表达远高于‘佐藤锦’。本研究对解释甜樱桃果实生长过程中色泽变化及推测Pac MYBA对甜樱桃果实花色苷起正调控作用提供理论基础。

For the fruit ripe to show different colors of Prunus avium（yellow variety Sato Nishiki, red varieties of Lapins）, The main components and contents of anthocyanins in fruit growth were determined by high performance liquid chromatography, a MYB gene of Sato Nishiki was cloned by RT-PCR, the expression of Pac MYBA gene in the growth of two varieties of Prunus avium was analyzed by real-time fluorescence quantitative PCR. The results indicated that the accumulation of anthocyanins after the conversion of Lapins fruit showed a significant upward trend, and anthocyanin components from a single one gradually rich for the three species. The content of anthocyanins after the conversion of Sato Nishiki was increased, but the increase was not significant and Anthocyanin is a single component. The relative expression of Pac MYBA in Lapins growth process was significantly increased and maintained at a higher level after veraison, the uptrend in Sato Nishiki goes gently and lower than Lapins. This research laid the theories basis of explaining the color change of Prunus avium fruit during growth and Pac MYBA plays a positive role in the regulation.