3,5-二硝基水杨酸法与酶法测定甜玉米还原糖和蔗糖含量的比较

Comparison of 3,5-Dinitrosalicylic Acid Method and Enzymatic Method in the Determination of Sugar and Sucrose Content in Sweet Corn

针对目前测定甜玉米还原糖和蔗糖含量的3,5-二硝基水杨酸法和酶法进行比较研究,分析两者在育种中的应用。结果显示:酶法的测量准确度比3,5-二硝基水杨酸法高,且其稳定性和重现性都优于3,5-二硝基水杨酸法。通过两种方法对10个甜玉米杂交种种子的还原糖和蔗糖含量的测定,证实了两种方法在甜玉米还原糖和蔗糖含量的检测上是一致的。从育种实践考虑,酶法适于样品数较小的检测,3,5-二硝基水杨酸法更适合于大批量样品的检测。

3,5-dinitrosalicylic acid method and enzymatic method are the general protocols in measuring the reducing sugar and sucrose contents in sweet corn. This study compared the 2 methods in breeding of sweet corn. The results showed that the measurement accuracy of enzymatic method was higher than that of 3,5-dinitrosalicylic acid method.The reproducibility and stability of enzymatic method were better than those of 3,5-dinitrosalicylic acid method. After measuring the contents of reducing sugar and sucrose of 10 sweet corn hybrid seeds by these 2 methods,it was confirmed that both 2 methods had consistent measurement results. From the breeding practice,enzymatic method was suitable for small quantity samples,while 3,5-dinitrosalicylic acid method was more suitable for measuring large number of samples.